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Food and Nutrition

Elisa Systems BLG Kit Evaluation -

Summary of results for individual laboratories and averages for all labs

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Baby Cereal Samples

Lab 1 Baby Cereal Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.5 - -0.2 -0.4 0.1
32 ppm 5 4.1 - 5.4 4.9 0.4
80 ppm 12.5 8.1 - 11.4 10.0 0.9

Lab 2 Baby Cereal Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.8 - -0.3 -0.6 0.1
32 ppm 5 5.7 - 6.8 6.1 0.4
80 ppm 12.5 6.7 - 16.1 12.2 3.2

Lab 3 Baby Cereal Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.3 - 0.0 -0.2 0.1
32 ppm 5 3.9 - 5.7 4.8 0.5
80 ppm 12.5 8.5 - 11.7 9.4 1

Lab 4 Baby Cereal Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.2 - 0.0 -0.2 0.1
32 ppm 5 3.9 - 5.1 4.6 0.3
80 ppm 12.5 9.5 - 12.1 10.7 0.8

Lab 5 Baby Cereal Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.1 - 0.3 0.2 0.2
32 ppm 5 3.8 - 4.7 4.2 0.3
80 ppm 12.5 8.6 - 10.0 9.4 0.5

Baby Cereal Results for all five labs
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.8 - 0.3 0.2 0.3
32 ppm 5 3.8 - 6.8 4.9 0.7
80 ppm 12.5 6.7 - 16.1 10.4 1.1

* based on designated reference material
** based on the kit calibrators

Cookie Samples

Lab 1 Cookie Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.2 - 0.5 -0.1 0.2
32 ppm 5 3.9 - 5.1 4.3 0.4
80 ppm 12.5 8.1 - 9.4 8.6 0.4

Lab 2 Cookie Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.7 - -0.2 -0.5 0.1
32 ppm 5 4.0 - 4.9 4.5 0.3
80 ppm 12.5 8.7 - 9.5 9.0 0.2

Lab 3 Cookie Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.6 - -0.4 -0.5 0.1
32 ppm 5 2.8 - 4.2 3.7 0.5
80 ppm 12.5 7.8 - 10.8 9.1 1

Lab 4 Cookie Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   0.0 - 0.1 0.0 0
32 ppm 5 3.9 - 5.6 4.5 0.5
80 ppm 12.5 8.9 - 10.7 9.7 0.5

Lab 5 Cookie Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   0.2 - 0.7 0.4 0.2
32 ppm 5 3.1 - 5.3 4.1 0.6
80 ppm 12.5 8.1 - 9.5 8.8 0.5

Cookie Results for all five labs
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.7 - 0.7 -0.1 0.4
32 ppm 5 2.8 - 5.6 4.2 0.3
80 ppm 12.5 7.8 - 10.8 9.0 0.4

* based on designated reference material
** based on the kit calibrators

Dark Chocolate Samples

Lab 1 Dark Chocolate Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.9 - -0.6 -0.3 0.5
32 ppm 5 2.0 - 3.9 3.1 0.7
80 ppm 12.5 5.5 - 7.3 6.6 0.7

Lab 2 Dark Chocolate Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.7 - -0.2 -0.4 0.2
32 ppm 5 2.6 - 4.3 3.2 0.5
80 ppm 12.5 6.3 - 7.8 6.9 0.5

Lab 3 Dark Chocolate Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.2 - -0.1 -0.2 0.1
32 ppm 5 2.8 - 3.4 3.1 0.2
80 ppm 12.5 6.9 - 7.7 7.2 0.3

Lab 4 Dark Chocolate Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.2 - 0.0 -0.4 0.2
32 ppm 5 2.2 - 3.6 2.9 0.4
80 ppm 12.5 6.5 - 7.5 6.9 0.3

Lab 5 Dark Chocolate Results
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.2 - 0.2 0.0 0.1
32 ppm 5 2.0 - 3.7 2.8 0.5
80 ppm 12.5 6.4 - 8.3 7.3 0.8

Dark Chocolate Results for all five labs
Spiking level* Expected Result** Range of Results Average Std Dev.
Blanks   -0.9 - 0.2 -0.2 0.2
32 ppm 5 2.0 - 4.3 3.0 0.1
80 ppm 12.5 5.7 - 8.3 7.0 0.3

* based on designated reference material
** based on the kit calibrators

Z -Scores for each Set of Samples

Commodity Spiking Level* ppm Expected Results** ppm Lab # Z-Score
Baby Cereal 0 0 1 -0.43
2 -1.31
3 0.08
4 0.22
5 1.43
Baby Cereal 32 5 1 -0.02
2 1.64
3 -0.17
4 -0.40
5 -1.05
Baby Cereal 80 12.5 1 -0.30
2 1.59
3 -0.80
4 0.30
5 -0.80

* based on designated reference material
** based on the kit calibrators

Commodity Spiking Level* ppm Expected Results** ppm Lab # Z-Score
Cookie 0 0 1 -0.39
2 3.00
3 2.61
4 -1.24
5 -3.98
Cookie 32 5 1 0.30
2 0.72
3 -1.54
4 0.93
5 -0.41
Cookie 80 12.5 1 -1.16
2 -0.03
3 0.22
4 1.52
5 -0.54

* based on designated reference material
** based on the kit calibrators

Commodity Spiking Level* ppm Expected Results** ppm Lab # Z-Score
Dark Chocolate 0 0 1 -0.39
2 -0.98
3 0.50
4 -0.62
5 1.50
Dark Chocolate 32 5 1 0.24
2 1.26
3 0.45
4 -0.62
5 -1.33
Dark Chocolate 80 12.5 1 -1.41
2 -0.17
3 0.80
4 -0.33
5 1.11

* based on designated reference material
** based on the kit calibrators