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Chemical Methods - Compendium of Methods for Chemical Analysis of Foods
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These methods for chemical analysis of foods were developed in the Food and Nutrition Research Laboratories of the Food Directorate, and in the Regional Laboratories, of Health Protection Branch (HPB), Health Canada. The methods are classified into Official Methods, HPB Methods, and Laboratory Procedures. Official Methods are those methods of analysis designated as such in the Food and Drugs Regulations. HPB Methods are fully validated by inter-laboratory studies and documented analytical methods. Laboratory Procedures are those methods validated by at least one HPB laboratory, but not fully validated by inter-laboratory studies.
This Compendium of Methods for Chemical Analysis of Foods is currently being updated to include new methods developed at the Food Research Division and the Regional Laboratories since 1995 and to reflect the formation of the new Health Products and Food Branch (HPFB).
For more information, please contact the Bureau of Chemical Safety.
See also: Compendium of Food Allergen Methodologies
| Identification Number | Title | Date Issued |
|---|---|---|
| Laboratory Procedures for Surveillance | ||
| LPS-001 | Laboratory Procedure for the Determination of Benzene in Beverages | June 5, 2006 |
| LPS-002 | Determination of Benzene in Soft Drinks and Other Beverages by Isotope Dilution Headspace Gas Chromatography/Mass Spectrometry | November 22, 2007 |
| LPS-003 | The Determination of Acrylamide in Foods by LC-ESI-MS-MS | January 8, 2008 |
| LPS-004 | Determination of Bisphenol A in Liquid Infant Formula by Solid Phase Extraction with Acetic Anhydride Derivatization and Gas Chromatography-Mass Spectrometry | August, 2008 |
| LPS-005 | Determination of Melamine in Various Milk-Containing Products | November, 2008 |
| Official Methods | ||
| FO-1 | Determination of protein rating | October 15, 1981 |
| FO-2 | Determination of volatile acidity of wine, cider and champagne cider | October 15, 1981 |
| FO-3 | Determination of carbon dioxide in baking powder | October 15, 1981 |
| FO-4 | Determination of arsenic in food colours | October 15, 1981 |
| FO-5 | Determination of lead in food colours | October 15, 1981 |
| FO-6 | Determination of higher aromatic hydrocarbons or tarry materials in carbon black and charcoal | October 15, 1981 |
| FO-7 | Determination of dye content of synthetic food colours | March 15, 1984 |
| FO-8 | Determination of water insoluble matter in synthetic food colours | March 15, 1984 |
| FO-9 | Determination of combined ether extracts in synthetic food colours | March 15, 1984 |
| FO-10 | Determination of subsidiary dyes in synthetic food colours | March 15, 1984 |
| FO-11 | Determination of intermediates in synthetic food colours | March 15, 1984 |
| FO-12 | Determination of volatile matter in Citrus Red No. 2 | March 15, 1984 |
| FO-13 | Determination of sulphated ash in Citrus Red No. 2 | March 15, 1984 |
| FO-14 | Determination of water soluble matter in Citrus Red No. 2 | March 15, 1984 |
| FO-15 | Determination of carbon tetrachloride insoluble matter in Citrus Red No. 2 | March 15, 1984 |
| FO-16 | Determination of tocopherol in milk fat or butter fat | October 15, 1981 |
| FO-17 | Extraction of soluble substances from vanilla beans | September 15, 1989 |
| FO-18 | Determination of drained tomato solids | October 15, 1981 |
| FO-19 | Determination of tomato solids | October 15, 1981 |
| FO-20 | Determination of drained solids of beans with pork or beans and pork and beans or vegetarian beans | October 15, 1981 |
| FO-21 | Determination of amino acids in grapefruit juice and orange juice | October 15, 1981 |
| FO-22 | Determination of potassium in grapefruit juice and orange juice | October 15, 1981 |
| FO-23 | Determination of absorbance value for total polyphenolics in grapefruit juice and orange juice | October 15, 1981 |
| FO-24 | Determination of Brix reading for grapefruit juice and orange juice | October 15, 1981 |
| FO-25 | Determination of acid in grapefruit juice or orange juice | October 15, 1981 |
| FO-26 | Determination of soluble solids in lemon juice, lime juice or lime fruit juice | October 15, 1981 |
| FO-27 | Determination of acid in lemon juice, lime juice or lime fruit juice | October 15, 1981 |
| FO-28 | Determination of starch in gluten flour | October 15, 1981 |
| FO-29 | Determination of moisture in grain | October 15, 1981 |
| FO-30 | Determination of ash in grain | October 15, 1981 |
| FO-31 | Determination of degree of fineness of grain | October 15, 1981 |
| FO-32 | Determination of fillers, binders and dextrose equivalent | October 15, 1981 |
| FO-33 | Determination of fat in meat and simulated meat products | October 15, 1981 |
| FO-34 | Determination of ash in edible bone meal or edible bone flour | October 15, 1981 |
| FO-35 | Determination of ash-free solids in gelatin | October 15, 1981 |
| FO-36 | Determination of wiener content of meat specialties | October 15, 1981 |
| FO-37 | Determination of water-soluble extractive in tea | October 15, 1981 |
| FO-38 | Determination of salt in smoked fish | March 15, 1985 |
| FO-39 | Determination of meat in boneless poultry | October 15, 1981 |
| FO-40 | Determination of vinyl chloride in food | October 15, 1981 |
| FO-41 | Determination of acrylonitrile in food | February 16, 1982 |
| FO-42 | Determination of bioavailability of iron | December 15, 1982 |
| HPB Methods | ||
| HPB-FC-1 | Determination of patulin by TLC | July 1981 |
| HPB-FC-2 | Determination of docosenoic acid in fats and oil by GLC | November 1981 |
| HPB-FC-3 | The determination of brominated vegetable oils in citrus-flavoured or spruce-flavoured beverages | November 1981 |
| HPB-FC-4 | Determination of fluoride in food by micro-diffusion using a fluoride-specific electrode | December 1981 |
| HPB-FC-5 | Determination of arsenic and selenium in foods (formerly LPFC-115) | May 1982 |
| HPB-FC-6 | Determination of N-nitrosodimethylamine in beer at the lower parts per billion level by a GLC-thermal energy analyzer technique | February 1982 |
| HPB-FC-7 | High performance liquid chromatographic determination of seven antioxidants in oil and lard (formerly LPFC-104) | April 1982 |
| HPB-FC-8 | Determination of N-nitrosodimethylamine in non-fat dry milk | January 1984 |
| HPB-FC-9 | Determination of tin in canned foods (formerly LPFC-112) | June 1984 |
| HPB-FC-10 | Determination of ethyl carbamate in table wine, sherry, liquors and liqueurs | April 1986 |
| HPB-FC-11 | Liquid chromatographic determination of domoic acid in mussels using the AOAC paralytic shellfish poison extraction procedure (formerly LPFC-151) | March 1989 |
| HPB-FC-12 | Rapid gravimetric method for analysis of water-soluble and insoluble dietary fibre (formerly LPFC-142) | January 1992 |
| HPB-FC-13 | Liquid chromatographic determination of nine antioxidants in butter oil | October 1992 |
| HPB-FC-14 | High pressure liquid chromatographic determination of Aflatoxins in peanut butter and other commodities (formerly LPFC-124) | June 1993 |
| Laboratory Procedures | ||
| LPFC-100 | Determination of monosodium glutamate in foods by GLC as trimethylsilyl derivative | September 1978 |
| LPFC-101 | Direct analysis of carbofuran and two non-conjugated metabolites in foods by high-pressure liquid chromatography with UV absorption detection | April 1978 |
| LPFC-102 | Determination of saccharin, sodium benzoate and caffeine in carbonated beverages by HPLC | April 1980 |
| LPFC-103 | Determination of parabenz in beverages by GLC | April 1979 |
| LPFC-105 | Determination of sorbic acid in fish | November 1979 |
| LPFC-106 | Determination of ethylenediaminetetraacetic acids (EDTA) in fish | November 1979 |
| LPFC-107 | Determination of ethoxylated mono- and diglycerides in bread | November 1979 |
| LPFC-108 | Determination of barban in wheat products | April 1980 |
| LPFC-109 | Comparison of liquid and gas chromatography for the determination of bromoxynil octanoate and benzoylprop-ethyl in wheat products | May 1980 |
| LPFC-110 | Determination of lead and cadmium in foods by atomic absorption spectrophotometry | August 1980 |
| LPFC-111 | Determination of ethoxyquin in spices | October 1980 |
| LPFC-114 | Rapid screening determination of lead in canned milk and infant formulas using graphite-furnace atomic absorption spectrometry | November 1980 |
| LPFC-116 | Rapid determination of lead and cadmium in foods using graphite-furnace atomic absorption spectrometry | April 1981 |
| LPFC-117 | Determination of deoxynivalenol (vomitoxin, DON) in wheat and other grains by gas chromatography with electron capture and mass spectrometric detection | January 1982 |
| LPFC-118 | Determination of metribuzin and its metabolites | March 1982 |
| LPFC-119 | Determination of triforine in fruit | March 1982 |
| LPFC-120 | Determination of Ergot alkaloids in wheat flour, rye flour and triticale flour | July 1982 |
| LPFC-121 | A rapid method for the simultaneous analysis of diquat and paraquat in potatoes | December 1982 |
| LPFC-122 | Determination of total lipids in foods | April 1983 |
| LPFC-123 | Determination of deoxynivalenol (vomitoxin, DON) in wheat flour by gas chromatography with electron capture and mass spectrometric detection | May 1983 |
| LPFC-125 | A rapid method for the determination of sodium and potassium | June 1983 |
| LPFC-126 | Colorimetric determination of nitrate and nitrite in foods | December 1983 |
| LPFC-127 | Screening test for the detection of sulphur dioxide in food products | April 1984 |
| LPFC-128 | Determination of sucrose diacetate hexaisobutyrate (SAIB) in soft drinks | April 1984 |
| LPFC-129 | Determination of egg-yolk solids in alimentary paste (formerly FA-1) | October 1984 Amended June 1985 |
| LPFC-130 | Modified babcock method for fat in ground meat using paley bottles (formerly FA-33) | October 1984 |
| LPFC-131 | Method for moisture in meat products (formerly FA-35) | October 1984 |
| LPFC-132 | Determination of the percentage nuts in mixed nuts or a mixture of nuts (formerly FA-80, may be modified) | |
| LPFC-133 | Determination of methyl bromide in foods | October 1984 |
| LPFC-134 | Determination of ethylene dibromide and other fumigants in foods | November 1984 |
| LPFC-135 | Enzymatic determination of polyunsaturated fatty acids (PUFA) | November 1984 |
| LPFC-136 | Determination of ethylene chlorohydrin in spices | March 1985 |
| LPFC-137 | Sample preparation by dry ashing for the determination of various elements by flame atomic absorption spectroscopy | April 1985 |
| LPFC-138 | Determination of nivalenol and deoxunivalenol (vomitoxin, DON) in cereals by gas chromatography with electron capture detection | June 1985 Amended November 1989 |
| LPFC-139 | High performance liquid chromatographic determination of seven antioxidants in dry foods, mayonnaise and salad dressings | December 1985 |
| LPFC-140 | Graphite-furnace atomic absorption spectrometric determination of lead and cadmium in foods after nitric-perchloric acid digestion and coprecipitation with ammonium pyrrolidine dithiocarbamate | December 1985 |
| LPFC-141 | Graphite-furnace atomic absorption spectrometric determination of arsenic in foods after wet-dry ash and coprecipitation with ammonium pyrrolidine dithocarbamate | February 1986 Amended January 1987 |
| LPFC-143 | Determination of diethylene glycol in wines by capillary gas chromatography | June 1986 |
| LPFC-144 | Determination of nivalenol, deoxynivalenol (vomitoxin, DON), T-2 toxin, HT-2 toxin and diacetoxyscirpenol in cereals by gas chromatography with electron capture detection | August 1986 |
| LPFC-145 | Gas chromatographic determination of polysaccharide gums in foods after hydrolysis and derivatization | December 1986 |
| LPFC-146 | HPLC analysis of diquat and paraquat in potatoes | January 1987 |
| LPFC-147 | An improved method for the determination of 1,1- dimethylhydrazine in apple and cherry products | August 1987 |
| LPFC-148 | The determination of naptalam and its metabolite in foods as 1-naphthylamine using HPLC and oxidative electrochemical detection | October 1987 |
| LPFC-149 | Determination of polycyclic aromatic hydrocarbons in foods by HPLC with fluorescence detection | August 1987 |
| LPFC-150 | Determination of zearalenone and a- and b-zearalenols in milk by liquid chromatography with fluorescence detection | December 1987 |
| LPFC-152 | Determination of fluazifop-butyl and fluazifop acid in soybeans and soybean oil using liquid chromatography with oxidative electrochemical detection | July 1988 |
| LPFC-153 | Determination of alachlor and its 2,6-diethylaniline-containing metabolites in corn, soybeans and potatoes as heptafluorobutyryl-2,6-diethylaniline | September 1988 |
| LPFC-154 | Determination of ergot alkaloids in wheat, rye and triticale flours, breads and bran | December 1988 |
| LPFC-155 | Determination of nivalenol, deoxynivalenol (vomitoxin, DON), T-2 toxin, HT-2 toxin, 15-monacetoxyscirpenol and diacetoxyscirpenol in wheat and soybean by gas chromatography with electron capture detection | July 1989 |
| LPFC-156 | Extraction and cleanup procedure for polychlorinated dioxins and furans in foods | November 1989 |
| LPFC-157 | Determination of ochratoxin A in meat by high performance liquid chromatography with fluorometric detection | November 1989 Amended December 1989 |
| LPFC-158 | Determination of sulfite in foods by liquid chromatography with amperometric detection | February 1990 |
| LPFC-159 | Determination of ethylenethourea (ETU) in fruit juices | May 1991 |
| LPFC-160 | Hydride generation atomic absorption spectrometric determination of selenium in foods | December 1990 Amended March 1991 |
| LPFC-161 | Determination of fumonisins B1 and B2 in corn and corn meal by high performance liquid chromatography with fluorometric detection | May 1991 Amended June 1991 |
| LPFC-162 | Sequential method for rapid determination of total dietary fibre | June 1992 |
| LPFC-163 | Determination of methyl carbamate and ethyl carbamate in alcoholic beverages and other fermented foods | May 1993 |
| LPFC-164 | Application of solid-phase microextraction to the headspace gas chromatographic analysis of halogenated volatiles in selected foods | October 1993 Amended December 1993 |
| LPFC-165 | Graphite-furnace atomic absorption spectrometric determination of total aluminum, copper, manganese, molybdenum and tin in infant formulas and evaporated milks | November 1994 |
| LPFC-166 | On-line steam distillation - purge and trap analysis of halogenated non-volatile contaminants in foods | March 1995 |
| LPFC-167 | Liquid chromatographic determination of okadaic acid and dinophysistoxin-1 in shellfish after derivatization with 9-chloromethylanthracene | June 1995 |
| LPFC-200 | The fluorometric determination of vitamin A in dairy products | December 1986 |
