Table II

Dilutions to be used and calculations of MPN per g or ml of test material

Dilutions*
		Undiluted	1:10	1:100	1:1000
Amount of original test material (g or mL)
	10	1	0.1	0.01	0.001	Combination to be used	MPN fromTable A-1	Dilutionfactor of middle dilution	MPN per mL or g
a	5/5**	5/5	2/5			5-5-2	540	1	5.4
b		5/5	5/5	2/5		5-5-2	540	10	54
c		5/5	5/5	2/5	2/5	5-2-2	95	100	95
d		5/5	5/5	2/5	0/5	5-2-0	49	100	49
e		2/5	2/5	1/5	0/5	2-2-1	12	10	1.2
f		5/5	2/5	1/5	1/5***	5-2-2	95	10	9.5
g		5/5	5/5	5/5	5/5	5-5-5	>1600	100	>1600

* Dilutions to be used are underlined.

** No. of positive tubes/No. of tubes inoculated.