Appendix G

Media Glossary
January 2006

Glossary of agars, broths and other reagents

Section F

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Fraser Broth (FB) (may also be designated as Modified Fraser Broth (MFB))

Basal Medium
Tryptose	10.0 g
Beef extract	5.0 g
Yeast extract	5.0 g
Sodium chloride (NaCl)	20.0 g
Mono-potassium phosphate (KH 2P0 4)	1.35 g
Na 2HPO 4	9.6 g
Esculin	1.0 g
Lithium chloride	3.0 g
Nalidixic acid	0.02 g
Acriflavine HCl	0.024 g
Distilled water	1.0 L
pH 7.2 ± 0.2
Supplement
Ferric ammonium citrate (FAC)	0.5 g
Distilled water	10.0 mL

Complete Medium:

Suspend the basal medium ingredients in 1.0 L of distilled water. Mix thoroughly. Heat to boiling to dissolve completely and autoclave at 121^oC for 15 min. Cool to room temperature. Asceptically add 10.0 mL of the FAC solution to the cooled media, mix well and dispense. Store under refrigeration in the dark.

FAC solution: suspend ferric ammonium citrate in 10.0 mL of distilled water and filter sterilize.

Note: acriflavine can photo-oxidize to form compounds inhibitory to Listeria sp