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|Beef extract||5.0 g|
|Yeast extract||5.0 g|
|Sodium chloride (NaCl)||20.0 g|
|Mono-potassium phosphate (KH 2P0 4)||1.35 g|
|Na 2HPO 4||9.6 g|
|Lithium chloride||3.0 g|
|Nalidixic acid||0.02 g|
|Acriflavine HCl||0.024 g|
|Distilled water||1.0 L|
|pH 7.2 ▒ 0.2|
|Ferric ammonium citrate (FAC)||0.5 g|
|Distilled water||10.0 mL|
Suspend the basal medium ingredients in 1.0 L of distilled water. Mix thoroughly. Heat to boiling to dissolve completely and autoclave at 121oC for 15 min. Cool to room temperature. Asceptically add 10.0 mL of the FAC solution to the cooled media, mix well and dispense. Store under refrigeration in the dark.
FAC solution: suspend ferric ammonium citrate in 10.0 mL of distilled water and filter sterilize.
Note: acriflavine can photo-oxidize to form compounds inhibitory to Listeria sp