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Appendix G
Media Glossary
January 2006
Glossary of agars, broths and other reagents
SECTIONS J-K
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(PDF Version - 18 K)| Basal Medium | |
|---|---|
| Proteose peptone No. 3 | 20.0 g |
| Potassium phosphate, dibasic, anhydrous (K2HPO4, ) | 1.5 g |
| Magnesium sulphate . 7 hydrate (MgSO4.7H2O) | 1.5 g |
| Glycerol | 15.0 mL |
| Agar | 20.0 g |
| Distilled water | 1.0 L |
| pH 7.2 ± 0.2 | |
Complete Medium:
Add the components to distilled water and bring the volume to 1.0 L. Mix thoroughly. Heat gently and bring to a boil. Dispense into tubes or flasks and autoclave for 15 min at 121°C. Pour into Petri dishes or leave in tubes.
| Basal Medium | |
|---|---|
| Agar* | 15.0 g |
| DL-Phenylalanine | 10.0 g |
| L-Ornithine-HCl | 10.0 g |
| Raffinose | 7.0 g |
| Pancreatic digest of casein | 2.5 g |
| Yeast extract | 2.5 g |
| Potassium phosphate, dibasic (K2HPO4) | 2.0 g |
| Phenol red solution (see below) | 10.0 mL |
| Distilled water | 990 mL |
| pH 5.6 ± 0.2 | |
| * Note: Additional agar can be added to inhibit the spread of mucoid colonies | |
| Supplements (1) Phenol Red Solution | |
| Phenol Red | 0.5 g |
| 50% Ethanol | 10.0 mL |
| Supplements (2) Carbenicillin | |
| Carbenicillin | 0.05 g |
| Distilled water | 10.0 mL |
Complete Medium:
Add components, except carbenicillin solution, to 990 mL distilled water. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min. Cool to 45-50°C. Aseptically add 10.0 mL of filter sterilized carbenicillin solution and mix thoroughly. Adjust pH and pour into sterile Petri dishes.
| Basal Medium | |
|---|---|
| Pancreatic digest of casein | 10.0 g |
| Peptic digest of animal tissue | 10.0 g |
| Lactose | 20.0 g |
| Glucose | 1.0 g |
| Sodium chloride (NaCl)* | 5.0 g |
| Ferric ammonium citrate | 0.5 g |
| Sodium thiosulfate (Na2S2Os) | 0.5 g |
| Agar | 15.0 g |
| Phenol red | 0.025 g |
| Distilled water | 1.0 L |
| pH 7.4 ± 0.2 | |
| *When used for halophilic Vibrio spp., add NaCl to a final concentration of 2-3%. | |
Complete Medium:
Mix ingredients thoroughly and heat with agitation to dissolve. Dispense into 13x100 screw-cap tubes and autoclave at 121°C for 15 min. Cool and slant to form deep butts.
