Health Canada
www.hc-sc.gc.ca
Common menu bar links
Appendix G
Media Glossary
January 2006
Glossary of agars, broths and other reagents
Sections Q-R
Help on accessing alternative formats, such as Portable Document Format (PDF), MP3 and WAV files, can be obtained in the alternate format help section.
(PDF Version - 15 K)| Basal Medium | |
|---|---|
| Brucella broth | 2.9 g |
| Sodium phosphate ( Dibasic) Na 2HPO 4 (anhydrous) | 0.118 g |
| Potassium phosphate ( Monobasic ) KH 2PO 4 (anhydrous) | 0.023 g |
| Agar | 0.2 g |
| Distilled water | 97.0 mL |
| pH about 7.3 | |
| Supplements | |
| A: 10% Ferrous sulphate.7 H 2O | 1.0 mL |
| B: 10% Sodium metabisulphite | 1.0 mL |
| C: 10% Sodium pyruvate | 1.0 mL |
Complete Medium:
Suspend basal medium ingredients in 97.0 mL of distilled water. Autoclave basal medium at 121oC for 15 min. Cool to 45oC. Prepare the supplement solutions separately in distilled water shortly before use and filter sterilize. Aseptically add 1.0 mL of solution A to 1.0 mL of solution B, mix well and add to 1.0 mL of solution C. Then add the whole mixture to the basal medium. Aseptically dispense 3.0 mL into sterile screw-capped tubes (13 x 100 mm). Prepare fresh media every two weeks.
| Basal Medium | |
|---|---|
| Sodium chloride (NaCl) | 7.2 g |
| Soya peptone | 4.5 g |
| Potassium phosphate ( Monobasic ) KH 2PO 4 | 1.26 g |
| Potassium phosphate ( Dibasic ) K 2HPO 4 | 0.18 g |
| Magnesium chloride (anhydrous) | 13.58 g |
| Malachite green | 0.036 g |
| Distilled water | 1.0 L |
| pH 5.2 ± 0.2 | |
Complete Medium:
Suspend basal medium ingredients into 1.0 L of distilled water. Heat gently to dissolve. Dispense into tubes and autoclave at 115oC for 15 min.
| Basal Medium | |
|---|---|
| Yeast extract | 3.0 g |
| Beef extract | 10.0 g |
| Peptone | 10.0 g |
| Soluble starch | 1.0 g |
| Dextrose | 5.0 g |
| Cysteine hydrochloride | 0.5 g |
| Sodium acetate, anhydrous | 3.0 g |
| Sodium chloride | 5.0 g |
| Agar (for solid medium) | 15.0 g |
| Distilled water | 1.0 L |
| pH 7.4 ± 0.2 | |
Complete Medium:
Suspend basal medium ingredients in 1.0 L of distilled water. Heat to boiling to dissolve ingredients. Adjust pH. Autoclave at 121oC for 15 min. Dispense into sterile Petri dishes.
