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Appendix G
Media Glossary
January 2006
Glossary of agars, broths and other reagents
Section V
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(PDF Version - 19 K)| Basal Medium | |
|---|---|
| Yeast extract | 3.0 g |
| Peptone | 7.0 g |
| Sodium chloride (NaCl) | 5.0 g |
| Bile salts no. 3 | 1.5 g |
| Lactose | 10.0 g |
| Neutral red | 0.03 g |
| Crystal violet | 0.002 g |
| Agar | 15.0 g |
| Distilled water | 1.0 L |
| pH 7.4 ± 0.2 | |
Complete Medium:
Suspend basal medium ingredients in 1.0 L of distilled water. Heat to boiling to dissolve completely. Do not boil for more than 2 min. Do not autoclave. Mix well before pouring. The medium should be prepared on the day of use. Prepared plates should not be incubated longer than 24 hours as the selectivity diminishes. Best results are obtained if plates are not too heavily seeded.
| Basal Medium | |
|---|---|
| Yeast extract | 3.0 g |
| Peptone | 7.0 g |
| Sodium chloride (NaCl) | 5.0 g |
| Bile salts no. 3 | 3.0 g |
| Lactose | 10.0 g |
| Neutral red | 0.06 g |
| Crystal violet | 0.004 g |
| Agar | 15.0 g |
| Distilled water | 1.0 L |
Complete Medium:
This formulation has double the usual concentrations of bile salts No. 3, neutral red and crystal violet.
Suspend basal medium ingredients in 1.0 L of distilled water. Heat to boiling to dissolve completely. Do not boil for more than 2 min. Do not autoclave. Mix well before pouring. The medium should be prepared on the day of use. Prepared plates should not be incubated longer than 24 hours as the selectivity diminishes. Best results are obtained if plates are not too heavily seeded.
| Basal Medium | |
|---|---|
| Yeast extract | 3.0 g |
| Peptone | 7.0 g |
| Sodium chloride (NaCl) | 5.0 g |
| Bile salts no. 3 | 1.5 g |
| Lactose | 10.0 g |
| Neutral red | 0.03 g |
| Crystal violet | 0.002 g |
| Agar | 15.0 g |
| Glucose | 10.0 g |
| Distilled water | 1.0 L |
| pH 7.4 ± 0.2 | |
Complete Medium:
Suspend basal medium ingredients in 1.0 L of distilled water. Heat to boiling to dissolve completely (no more than 2 min). Do not autoclave. Cool and dispense. If not used immediately, store in a refrigerator.
| Reagent A | |
|---|---|
| a-naphthol | 5.0 g |
| Ethanol (absolute) | 100 mL |
| Reagent B | |
| Potassium hydroxide (KOH) | 40.0 g |
| Distilled water | 100 mL |
Complete Medium:
Reagent A: Dissolve a -naphthol in 100 mL of ethanol. Add 0.6 mL of inoculated medium. Shake.
Reagent B: Dissolve KOH in 100 mL of distilled water. Cool and adjust volume. Add 0.2 mL to inoculated medium. Shake.
Addition of a few crystals of creatine to the test medium may intensify and speed reactions. A positive test is indicated by the development of a pink-red colour within 5-10 min .
