Appendix G

Media Glossary
January 2006

Glossary of agars, broths and other reagents

Sections X-Y

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Xylose-Lysine-Deoxycholate (XLD) Agar

Basal Medium
Lactose	7.5 g
Sucrose	7.5 g
Sodium thiosulfate (Na 2 S 2 O 3)	6.8 g
L-Lysine	5.0 g
Sodium chloride (NaCl)	5.0 g
Xylose	3.75 g
Yeast extract	3.0 g
Sodium deoxycholate (C 24H 39O 4Na)	2.5 g
Ferric ammonium citrate	0.8 g
Phenol red	0.08 g
Agar	15.0 g
Distilled water	1.0 L
pH 7.4 ± 0.2

Complete medium:

Suspend basal medium ingredients in 1.0 L of distilled water. Mix thoroughly and gently heat with agitation just until the medium boils. Do not overheat. DO NOT AUTOCLAVE. Overheating causes precipitation. Cool to 45-50^oC and dispense into sterile Petri dishes.