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|Sodium thiosulfate (Na 2 S 2 O 3)||6.8 g|
|Sodium chloride (NaCl)||5.0 g|
|Yeast extract||3.0 g|
|Sodium deoxycholate (C 24H 39O 4Na)||2.5 g|
|Ferric ammonium citrate||0.8 g|
|Phenol red||0.08 g|
|Distilled water||1.0 L|
|pH 7.4 ▒ 0.2|
Suspend basal medium ingredients in 1.0 L of distilled water. Mix thoroughly and gently heat with agitation just until the medium boils. Do not overheat. DO NOT AUTOCLAVE. Overheating causes precipitation. Cool to 45-50oC and dispense into sterile Petri dishes.