Health Canada
www.hc-sc.gc.ca
Common menu bar links
Institutional links
Appendix G
Media Glossary
January 2006
Glossary of agars, broths and other reagents
Section A
Help on accessing alternative formats, such as Portable Document Format (PDF), MP3 and WAV files, can be obtained in the alternate format help section.
(PDF Version - 18 K)| Basal Medium | |
|---|---|
| Proteose peptone | 5.0 g |
| Yeast Extract | 5.0 g |
| Dextrose | 5.0 g |
| Potassium phosphate, dibasic (K 2HPO 4) | 4.0 g |
| Distilled water | 1. 0 L |
Complete Medium
Adjust the pH of the medium to the pH of the food being analysed with 1N HCl solution. Sterilize at 121oC for 20 min.
| Basal Medium | |
|---|---|
| Sodium Thiosulfate (Na 2S 2O 3) | 10.67 g |
| Proteose peptone | 5.0 g |
| Sodium chloride (NaCl) | 5.0 g |
| Xylose | 3.75 g |
| L-Lysine.HCl | 3.5 g |
| Yeast extract | 3.0 g |
| Sorbitol | 3.0 g |
| Bile salts No.3 | 3.0 g |
| Inositol | 2.5 g |
| L-Arginine.HCl | 2.0 g |
| Lactose | 1.5 g |
| Ferric ammonium citrate | 0.8 g |
| Bromthymol blue | 0.04 g |
| Thymol blue | 0.04 g |
| Agar | 12.5 g |
| Distilled water | 1.0 L |
| pH 8.0 ± 0.1 | |
| Supplement | |
| Ampicillin | 5. 0 mg |
Complete Medium:
Add components, except ampicillin, to distilled water. Mix thoroughly. Gently heat and bring to a boil. Do not autoclave. Cool to 50oC. Aseptically add 5.0 mg of ampicillin and dispense.
Note: The supplement is commercially-available.
| Basal Medium | |
|---|---|
| Peptone | 15.0 g |
| Yeast extract | 4.0 g |
| Sodium chloride (NaCl) | 5.0 g |
| Distilled water | 970 mL |
| pH 7.4 ± 0.2 | |
| Supplement | |
| Sodium Bicarbonate (NaHCO 3), 10% aqueous, filter sterilized | 30.0 mL |
Complete Medium
Prepare on day of use. Dissolve first 3 ingredients in distilled water. Autoclave 15 min at 121oC . Cool then add 30 mL NaHCO 3. Mix and aseptically dispense into screw-cap tubes (15.0 mL for 16x125 mm tubes).
| Basal Medium | |
|---|---|
| Peptone | 10.0 g |
| Sodium chloride (NaCl) | 30. 0 g |
| Distilled water | 1.0 L |
| pH 8.5 ± 0.2 | |
Complete Medium:
Add components to distilled water. Mix thoroughly. Adjust the pH so that the value after sterilization is 8.5 ± 0.2. Dispense 10 mL into tubes and autoclave 10 min at 121oC.
| Basal Medium | |
|---|---|
| Peptone | 10.0 g |
| Sodium chloride (NaCl) | 10.0 g |
| Distilled water | 1.0 L |
| pH 8.5 ± 0.2 | |
Complete Medium:
Add components to distilled water. Mix thoroughly. Adjust the pH so that the value after sterilization is 8.5 ± 0.2. Dispense and autoclave 10 min at 121oC.
| Basal Medium | |
|---|---|
| Peptone | 5.0 g |
| Yeast extract | 3.0 g |
| Tryptone | 10.0 g |
| Sodium chloride ( NaCl ) | 20.0 g |
| Glucose | 1. 0 g |
| L-Arginine (hydrochloride) | 5.0 g |
| Ferric ammonium citrate | 0.5 g |
| Sodium thiosulfate | 0.3 g |
| Bromcresol purple | 0.02 g |
| Agar | 13.5 g |
| Distilled water | 1.0 L |
| pH 6.9 ± 0.1 | |
Complete Medium:
Add ingredients to distilled water. Mix thoroughly and heat to dissolve. Dispense into tubes and autoclave at 121o C for 10-12 min. Allow tubes to cool in a slanted position.
