1. Application

The following information is offered as a supplement to all the methods in The Compendium of Analytical

Methods, and may be used with any method, unless otherwise specified. The purpose of this supplement is to provide additional information (such as source(s) of materials, critical steps, confirmation steps, or helpful hints etc.).

7. Procedure

Preparation of sample

The initial 1:10 dilution can be prepared by using either weight to volume or weight to weight ratios. For example, most methods state: "prepare a 1:10 dilution of the food by adding aseptically 11(10) g (the analytical unit) to 99(90) mL of the required diluent as indicated. If five sub-samples are composited for analysis, aseptically blend or stomach 50 g or mL into 450 mL of the required diluent". Other analytical sizes may be used as long as the 1:10 ratio is maintained.

Alternately, prepare a 1:10 dilution of the food by adding aseptically 11(10) g (the analytical unit) to 99(90) g of the required diluent. Other analytical sizes may be used as long as the 1:10 ratio is maintained.