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Summary of Methods in this Compendium that Detect the Presence of Listeriaspp. and Listeria Monocytogenes

Health products and food branch - Ottawa

Method Summary
January 2005

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NOTE: The following is a summary of methods in the Compendium of Analytical Methods that can be used to detect the presence of Listeria spp. and Listeria monocytogenes. This summary can be used to aid the Laboratory Supervisor and Analyst in choosing the appropriate methods for the analysis of their sample(s). It is NOT an indication that each method must be used for each sample.

It is the responsibility of the Lab Supervisor to ensure that the latest version of each method and Supplements (as appropriate) are being used.

Method Type	Identification Number of Method and Supplements	Title
Isolation and Identification	MFHPB-07	The Detection of Listeria spp. in Foods and Environmental Samples using Palcam Broth.
	MFHPB-30	Isolation of Listeria monocytogenes from All Foods and Environmental Samples.
		Supplement to Method MFHPB-30, March 2002
		Supplement to Method MFHPB-30, September 2002
	MFLP-78	Detection of Presumptive Positive Listeria monocytogenes from Foods and Environmental Samples by the Polymerase Chain Reaction (PCR)
Enumeration (cultural confirmation required)	MFLP-74	Enumeration of Listeria monocytogenes in foods
Enumeration (cultural confirmation required)	MFLP-74	Supplement to the Method MFLP-74 April 2004
Immunological Screening (cultural confirmation required)	MFHPB-29	Detection of Listeria spp. in Foods and Environmental Samples by the VIDAS Listeria^TM Method
	MFLP-33	Detection of Listeria monocytogenes in Foods and Environmental Samples by the VIDAS LMO 2^TM Method
	MFLP- 34	Detection of Listeria spp. in Foods and Environmental Samples by the VIP Method
	MFLP-71	Detection of Listeria spp. in Foods Using the Oxoid Listeria Rapid Test (The Clearview Kit)
	MFLP-99	Detection of Listeria spp..in Foods and Environmental Samples by the EiaFoss Listeria ^TM Method
Molecular Screening (cultural confirmation required)	MFLP-28	The QUALICON BAX^® SYSTEM Method for the Detection of Listeria monocytogenes in a Variety of Food
	MFLP-28	Supplement to Method MFLP-28 Jan. 2004
	MFLP-31	Identification of Listeria monocytogenes by the Genevision Real Time Polymerase Chain Reaction System
	MFLP-88	Identification of Listeria monocytogenes by Accuprobe^TM