Health Canada
Symbol of the Government of Canada
Food and Nutrition

Official Methods for the Microbiological Analysis of Foods

Table of Contents - Volume 1 - The Compendium of Analytical Methods
Identification
Number
Title Date Issued
Preface Preface to the Compendium of Analytical Methods January 2003
Disclaimer Disclaimer for the Compendium of Analytical Methods January 2005
Terms of Reference Terms of Reference for the Microbiological Methods Committee April 2009
Interpretive Summary Health Products and Food Branch Standards (HPFB) and Guidelines for Microbiological Safety of Food - Interpretive Summary April 2008
Ex.Mat. Overview Guidelines for the General Cleanliness of Foods - An Overview March 2009
Development of Methods Procedure for the Development and Management of Food Microbiological Methods April 2008
Guidelines for Modifying or Revoking Food Microbiological Methods July 2012
Part 1. Definitions and Explanations of Methods and General Procedures April 2008
Part 2 (A). Data Requirements, Submission Templates and Examples for Quantitative Methods May 2008
Part 2 (B). Data Requirements, Submission Templates and Examples for Qualitative Methods. June 2008
Part 3. Guidelines for the Evaluation of Quantitative Food Microbiological Methods.  
Part 4: Guidelines for the Relative Validation of Indirect Qualitative Food Microbiological Methods. March 2011
Part 5: Guidelines for the General Requirements to Verify Validated Food Microbiological Methods for Implementation in Routine Testing.  
Part 7: Validation of Platform Upgrades for Rapid Test Methods December 2011
Guidelines PCR Technology BMH Guidelines on the Use of PCR Technology for the Detection of Bacterial Foodborne Pathogens Archived September 2012
MFO-2 Microbiological Examination of Ice Cream and Ice Milk November 30, 1981
MFO-3 Determination of Phosphatase Activity in Dairy Products November 30, 1981
MFO-4 Microbiological Examination of Cottage Cheese November 30, 1981
MFO-5 Examination of Canned Tomatoes, Tomato Juice and Vegetable Juice, Tomato Puree, Tomato Paste, Tomato Pulp and Tomato Catsup for Mould Filaments November 30, 1981
MFO-6 Microbiological Examination of Egg Products and of Liquid Eggs November 30, 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-7 Microbiological Examination of Milk November 30, 1981
MFO-8 Determination of Sediments in Milk November 30, 1981
MFO-9 Microbiological Examination of Mineral Water November 30, 1981
MFO-10 Microbiological Examination of Frog Legs November 30, 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-11 Microbiological Examination of Cocoa and Chocolate November 30, 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-12 Microbiological Examination of Milk Powder November 30, 1981
Supplement to All Official Methods (MFO-6, MFO-10, MFO-11 and MFO-12) That Detect Salmonella March 2009
MFO-14 Microbiological Examination of Cheese November 30, 1983
Note of Interpretation for Method MFO-14 April 2012
MFO-15 Microbiological Examination of Water in Sealed Containers (excluding Mineral and Spring Water) and of Prepackaged Ice November 30, 1981
Appendix A Definitions of Terms November 2006
Appendix B Collection and Transportation of Samples November 2006
Appendix C Calculation of the Most Probable Number of Growth Units for HGMF Methods March 1993
Appendix D Calculation of the Most Probable Number (MPN) March 1993
Appendix E Enzyme Treatment of Foods for Filtration September 1993
Appendix F Supplement to all the Methods in the Compendium:
the use of a Stomacher vs a blender
November 1999
Appendix G Glossary of Agars, Broths and Other Reagents  
Index and Introduction February 2006
Section A January 2006
Section B January 2006
Section C January 2006
Section D January 2006
Section E January 2006
Section F January 2006
Section G January 2006
Section H January 2006
Section I January 2006
Sections J-K January 2006
Section L January 2006
Section M January 2006
Section N January 2006
Section O January 2006
Section P February 2006
Sections Q-R January 2006
Section S January 2008
Section T February 2006
Section U January 2006
Section V January 2006
Section W January 2006
Sections X-Y January 2006
Appendix H Preparation of Dilutions using Gravimetric Means December 2001
Appendix I Supplement to All Methods in the Compendium: General Microbiological Guidance

Supplement to Appendix I
January 2003

June 2005
Appendix J Supplement to All Methods That Detect Salmonella November 2003
Appendix K Summaries of Methods that Detect Specific Bacteria in this Compendium  
E. coli O157:H7 March 2012
Listeria spp. and L. monocytogenes March 2012
Staphylococcus aureus March 2012
Salmonella spp. March 2012
Appendix L Confirmation Steps for Methods for The Detection of Listeria spp. In Foods And Environmental Samples Archived July 2012

Evaluation Division
Bureau of Microbial Hazards
Food Directorate
Health Products and Food Branch
Health Canada
4th Floor N.W. SFBRC [2204A1]
Ottawa, Ontario K1A 0L2
Telephone: (613) 957-0349
Fax: (613) 952-6400
Micro_methods_committee@hc-sc.gc.ca