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Food and Nutrition

Appendix G

Media Glossary
February 2006

GLOSSARY OF AGARS, BROTHS AND OTHER REAGENTS

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PDF Version

INDEX

Section A

Acid Broth (AB)

Aeromonas Medium (Ryan's)

AKI Medium

Alkaline Peptone Salt Broth (APS)

Alkaline Peptone Water (APW)

Arginine Glucose Slant (AGS)

Section B

Baird-Parker (BP) Medium

Bismuth Sulfite Agar (BS)

Blood Agar - (Trypticase Blood Agar Base)

Blood Agar- Listeria

Brain Heart Infusion (BHI) Broth

Brain Heart Infusion Broth with 0.6% Yeast Extract (BHI-YE)

Brilliant Green-lactose Bile (2%) (BGLB) Broth

Brilliant Green Sulfa Agar (BGS)

Brilliant Green Water (0.002% w/v)

Brucella Broth

Brucella Semi-solid Medium

Buffered Glucose Broth (MR-VP medium)

Buffered Glucose Salt Broth (MR-VP medium)

Buffered Peptone Water (BPW)

Section C

Campylobacter Agar with Charcoal and Deoxycholate (CCDA)
(Also known as: Modified CCDA Preston)

Carbohydrate Fermentation Agar (Rhamnose, Mannitol and Xylose) (for Listeria identification)

Carbohydrate Fermentation Broth (for Listeria identification)

Cary and Blair Transport Media

Casamino Acids-Yeast Extract (CYE) Broth

Cetrimide Agar (Pseudosel® Agar)

Coagulase Plasma

Cooked Meat Medium (CMM)

Crystal Violet (Hucker's)

Section D

Decarboxylase Basal Medium (Arginine, Lysine, Ornithine)

D/E Neutralizing Agar

Dichloran Rose Bengal Chloramphenicol (DRBC) Agar

Dichloran-glycerol (DG18) Agar

Disinfecting solution for containers - chlorine

Section E

EC (E. Coli) Broth

EC-MUG (4-methylumbelliferyl-β-D-glucuronide) Broth

EF-18

EHEC Enrichment broth (EEB)

EIA (V. vulnificus) Wash Solution

ELISA Buffer (for Cholera Toxin Test)

ENDO Agar

Section F

Fraser Broth (FB) (May also be designated as Modified Fraser Broth (MFB))

Section G

Gelatin Agar (GA)

Gelatin Phosphate Buffer (Gelatin Phosphate Diluent )

Gelatin Salt Agar (GSA)

Glycerol-peptone Medium

Gram Negative Broth (GN Broth or Hajna GN Broth)

Section H

Heart Infusion (HI) Broth and Agar (HIA) (for Vibrio)

Hektoen Enteric Agar

Hemolytic Ceftazidime-Lithium Chloride Agar (HCLA)

HC (Hemorrhagic Coli) Agar

Hippurate Hydrolysis Testing Reagents

Hugh-Leifson Glucose Broth (HLGB)

Section I

Indole Reagent (Kovac's)

Indole Reagent (for MFHPB-27)

Iodine Solution (Gram's)

Iodine Solution (Lugol's)

Section J-K

King's Medium B

Klebsiella Selective Agar

Kligler Iron Agar (KIA)

Section L

Lactose Broth (LAC)

Lactose Gelatin (LG)

Lauryl Sulfate Tryptose (LST) Broth

LB Agar, Miller (Luria-Bertani) Agar

Levine's Eosin Methylene Blue (L-EMB) Agar

Listeria Enrichment Broth (LEB) (UVM formulation)

Lithium Chloride-Phenylethanol-Moxalactam Medium (LPM)

Liver Veal Agar (LVA)

Liver Veal Egg-yolk Agar

Long-term Preservation Medium (for Vibrio spp)

Lysine Iron Agar (LIA)

Lysostaphin Solution

Section M

M Broth

MacConkey Agar

Malachite Green (5%)

Malt Extract

mA-Mannitol (MAM) Agar

Mannitol Maltose Agar

mA-Trehalose (MAT) Agar

Methyl Red test reagent

Microbial Content Test Agar (also known as Tryptic Soy Agar with Lecithin and Polysorbate 80 [TSALT], and Casein Soy Peptone with Polysorbate 80 and Lecithin)

m-FC agar (without rosolic acid)

Milk agar

Modified CCDA Preston - see Campylobacter Agar with Charcoal and Deoxycholate (CCDA)

Modified Cellobiose-Polymyxin B - Colistin Agar (mCPC)

Modified E. coli Broth with Novobiocin (mEC-n)

Modified Fraser Broth (MFB) - see Fraser Broth

Modified HC Agar (mHC)

Modified Oxford Agar (MOX)

Modified PE-2 Medium (PE2)

Modified Semi-Solid Rappaport Vassiliadis (MSRV) Medium

Modified Sorbitol MacConkey Agar (TCCSMAC)

Modified TSB with Novobiocin (mTSB-n)

Motility Test Medium

Motility Test Medium with 2,3,5-triphenyltetrazolium chloride (TTC)

mPAC Agar

MR-VP medium - See Buffered Glucose Broth and Buffered Glucose Salt Broth

Mueller-Hinton Agar (MH)

Mueller-Hinton Agar with Blood

Section N

Neutralizing Buffer

Nitrate Motility (Nm) Agar (Motility-Nitrate Medium, Buffered for C. perfringens)

Nitrate Reagent

Nutrient Agar

Nutrient Broth (NB)

Section O

O-Nitrophenyl-beta-D-galactoside (ONPG)

Orange Serum Agar (OSA)

Oxford Agar (OXA)

Oxidase Reagent

Oxidative-Fermentative (OF) Glucose Media , Semisolid

Oxytetracycline Glucose Yeast Extract Agar (OGYE)

Section P

Palcam Agar (PAL)

Palcam Broth

Park and Sanders' Enrichment Broth

Penicillin-Streptomycin Solution

Peptone (1.0%) Salt Diluent

Peptone Salt Nitrate Broth

Peptone (0.1%) Water Diluent

Peptone/Tween 80 diluent

Peroxidase Substrate Solution (ABTS)
(2,2"-Azino-di-[3-ethyl benzthiazoline sulfonate (6)] (ABTS substrate)

Peroxidase Substrate Solution (4-chloro-1- naphthol) for Membrane ELISA

Phenol Red Carbohydrate Broth (0.5%)

Phenol Red Sorbitol Agar with MUG

Phosphate Buffer (Butterfield's)

Phosphate Buffered Saline (PBS) (pH 7.3-7.4, 0.02 M)

Phosphate Buffered Saline (PBS for Ridascreen)

Plate Count Agar (PCA)

Plate Count Agar with Chloramphenicol

Polymyxin Pyruvate Egg Yolk Mannitol Bromthymol Blue Agar (PEMBA MEDIUM)

Potato Dextrose Agar (PDA)

Potato Dextrose Agar with Chloramphenicol

Potato Dextrose - Salt Agar

Potato Dextrose Agar (Acidified) (PDA)

Presence-Absence Broth

Preston Agar (Campylobacter Selective Medium)

Pseudomonas-CFC Agar

Pseudomonas-CN Agar

Purple broth base with cellobiose

Section Q-R

Rapid H2S Test Medium

Rappaport-Vassiliadis Soya Peptone Broth (RVS)

Reinforced Clostridial Medium (RCM)

Section S

Sabouraud Dextrose Agar (SDA)

Safranin (0.5%)

Safranin counterstain (Hucker's)

Saline, physiological

Selenite Cystine Broth (SC)

Serum-inositol Medium (for lyophilization of cultures)

Shigella Broth

SIM Medium (also known as: Sulfide Indole Motility Medium)

Simmons Citrate Agar

Skim Milk Medium

Sodium Chloride (0% NaCl)-Peptone Broth

Sodium Chloride (NaCl)-peptone Broth, 3% (also 7% and 10%)

Sodium Citrate, aqueous (2%)

Sorbitol - Bile Salts Broth

Sorbitol MacConkey Agar

Sorbitol MacConkey Agar with BCIG

Sudan Black B

Sulfite Cycloserine Agar (originally designated EY free Tryptose Sulfite Cycloserine (TSC) Agar)

Section T

T1N0, T1N1 T1N2, T1N3 Medium - See Tryptone Salt Agar

Tetracycline, 0.2% stock solution

Tetrathionate Brilliant Green Broth (TBG)

Thermoacidurans Agar (TA)

Thiosulphate Citrate Bile Salts Agar (TCBS) (Oxoid designation is Cholera Medium TCBS)

Toluidine Blue DNA Agar

Trimethylamine N-oxide (TMAO) Medium

2,3,5, Triphenyltetrazolium Chloride (TTC) 1% Solution

Triple Sugar Iron Agar (TSI)

Triple Sugar Iron NaCl Agar

Tris Buffer, 0.05M, pH 9.0

Tris-buffered Saline (TBS) (pH 7.5)

Tris-buffered Saline with 1% Gelatin

Tris-buffered Saline with 3% Gelatin

Tris-buffered Saline (TBS) - Tween

Trypsin-EDTA Solution, 1X

Trypsin solutions

Trypticase Peptone Glucose Yeast Extract (TPGY) Broth

Tryptic Soy Agar (TSA) (Soybean Casein Digest Agar) (ATCC Medium 77)

Trypticase Soy Broth (TSB) (Soybean Casein Digest Broth)

Tryptic Soy Broth (TSB) with Glycerol (extra NaCl and without dextrose)

Tryptic Soy-Fast Green Agar (TSFA)

Tryptic Soy-Magnesium Sulfate Agar (TSAM)

Trypticase Soy Agar with 0.01% TTC

Trypticase Soy Agar with 0.6% Yeast Extract (TSA-YE)

Trypticase Soy Broth with 0.6% Yeast Extract (TSB-YE)

Tryptone Broth (1%)

Tryptone Bile Agar (TBA)

Tryptone Broth and Tryptone Salt Broths (T1N0, T1N1 T1N3, T1N6, T1N8, T1N10)

Tryptone Salt (T1N1) Agar (also T1N0, T1N2, T1N3)

Tryptose Broth and Agar for Confirmation Tests and Serology

Section U

Universal Preenrichment Broth

Urea Agar (Christensen's)

UVM1 and UVM 2 (University of Vermont Listeria Enrichment Broth) - see Listeria Enrichment Broth

Section V

Violet Red Bile Agar (VRBA)

Violet Red Bile Agar (Double Strength )

Violet Red Bile Glucose Agar

Voges-Proskauer (V-P) test reagents

Section W

Wagatsuma agar (for Kanagawa test)

Wilkins Chalgren Anaerobe Blood Agar

Wilkins Chalgren Anaerobe Broth

Section X-Y

Xylose-Lysine-Deoxycholate (XLD) Agar

INTRODUCTION

The following glossary is provided as a handy reference for agars, broths and other reagents used in The Compendium of Analytical Methods. It is the responsibility of the analyst or laboratory supervisor to ensure the efficacy of media prepared from commercially available products or made from scratch using the formulas below or variations of these formulas.

It is the responsibility of the analyst or laboratory supervisor to ensure that proper precautions are taken when handling the chemical components of media, including the commercially prepared product, to avoid potential injury due to inhalation, contact with skin and eyes, as well as effects of long term exposure.

Notes (GENERAL):

  1. pH: Unless otherwise indicated, pH determinations are performed at 25°C prior to heating the media.

  2. Distilled water: The term ' distilled water' has been used in the formulas described below for simplicity and deNotes the use of reagent-grade water in general. Water purification systems will vary between laboratories. It is the responsibility of each laboratory to ensure the quality of their reagent water.

  3. Media Formulas: Media formulas and the pH of the reconstituted product mary vary slightly from manufacturer to manufacturer, as individual components names may vary or be substituted for similar components (i.e. different protein formulations). In some cases additional components may be included in the formula or others substracted. If the method or glossary indicates that commercial products can be used, then it up to the Laboratory Supervisor to ensure that the formula used performs according to the method. The pH of reconstituted commercial products must conform to that stated by the manufacturer.
Date Modified: 2006-02-28

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