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1.3 Table for Quantitative Method - Comparative / Collaborative Study Data

Instructions for completing table

Details of the following should be provided in written protocols.
In the following tables differentiate Comparative data (Method comparison study) from data obtained from Collaborative studies

The following statisticical analyses are needed (as applicable) linearity, CL, LOD and LOQ. See Part 3 of this document.

Submit statistical analysis reports as additional documents (in-house or published peer-reviewed statistical reports )

Numbers below correlate to those in the table :

1. Alternative method(s): List the name of the method(s) being compared to the reference method.
2. Reference method: List the name of the standard reference method.
3. Diluents and agars used. List broths and incubation times and temperatures for each. List any variations that may be specific to a food category .
4. Food category: list whether it is (1) Processed meat products (2) Raw meat products (3) Poultry products (4) Eggs and derivatives (5) Fish and Seafood Products (6) Fruits and vegetable based products (7) Dairy Products (8) Chocolate and bakery Products, (9) Animal Feed (10) Others (e.g. Cereals, Spices, Processed foods (e.g. Canned products, salad dressings, etc.)
5. Food Types: list the sample type within a sample category such as ground beef, chicken wings, or minced pork etc.
6. Naturally-contaminated (NC) or artificially-contaminated (AC)? Must include contamination level for AC, as CFU/25 g or CFU/g, as well as describe the AC process in the protocol (including stressing, allowing the sample to age or equilibrate etc.). It is preferable to include contamination level for NC if available.
7. Plating results: include data on novel media as well as the reference agar(s). Do a Log 10 conversion.
8. Confirmation: describe the details of the biochemicals used in the protocol. In the table indicate if typical (+) or negative (-). Give details if the biochemicals are atypical (i.e., H2S-). For some species, an acronym could be used, e.g., biochemicals typical for Listeria monocytogenes (LM) or other Listeria spp. (L).
9. Additional confirmation tests: list any novel biochemicals if part of the submission with results. Give details of these in the protocol.
10. Comments: provide any additional details or facts that may aid in the review.

Alternative Method Comparative Study:

1. Alternative Method(s):
2. Reference Method:
3. Diluents and agars used: