Health Protection Branch - Ottawa
Official Method MFO-4
November 30, 1981
Help on accessing alternative formats, such as Portable Document Format (PDF), Microsoft Word and PowerPoint (PPT) files, can be obtained in the alternate format help section.
- 1 -
This method shall be used for the determination of coliform bacteria (Coliforms) in cottage cheese in accordance with Section B.08.054 of the Food and Drug Regulations.
2.1.1 Lot: A batch or production unit which may be identified by the same code. When there is no code identification, a lot may be considered as (a) that quantity of cottage cheese produced under essentially the same conditions, at the same establishment and representing no more than one day's production; or, (b) the quantity of the same variety of cottage cheese from one and the same manufacturer available for sampling at a fixed location.
2.1.2 Sample: The sample units (subsamples) taken from a lot for analysis.
2.1.3 Sample Unit: Usually a consumer size container of the product, and should consist of a minimum of 100 g. A sample unit is often referred to as a subsample.
2.1.4 Analytical Unit: That amount of product withdrawn from the sample unit for analysis.
2.2.1 A sample, consisting of five sample units drawn at random from each lot, shall be taken.
2.2.2 Each sample unit shall consist of at least 100 g.
2.2.3 Collect original unopened containers wherever possible.
2.2.4 More than one sample unit may be collected from large institutional or bulk containers when the total number of sample units required exceeds the number of containers in the lot. When the lot consists of containers smaller than 100 g, a sample unit will consist of more than one containers (e.g., four 25 g containers in each sample unit).
2.2.5 Employ aseptic techniques in collecting the sample units when sampling from bulk. Place each collected sample unit into a separate sterile container.
2.2.6 Keep sample units refrigerated (0-5°C) during transport.