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Food and Nutrition

Microbiological Examination of Mineral Water

Health Protection Branch - Ottawa

Official Method MFO-9
November 30, 1981

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1. APPLICATION

This method shall be used for the determination of coliform bacteria (Coliforms) in mineral water in accordance with Section B.12.001(b) of the Food and Drug Regulations.

2. SAMPLING

2.1 Definition of Terms

2.1.1 Lot: A batch or production unit which may be identified by the same code. When there is no code identification, a lot may be considered as (a) that quantity of mineral water produced under essentially the same conditions, at the same establishment and representing no more than one day's production; or, (b) the quantity of mineral water from one and the same manufacturer available for sampling at a fixed location.

2.1.2 Sample: The sample units (subsamples) taken from a lot for analysis.

2.1.3 Sample Unit: Usually a consumer size container of the product, and should consist of a minimum of 100 ml. A sample unit is often referred to as a subsample.

2.1.4 Analytical Unit: That amount of product withdrawn from the sample unit for analysis.

2.2 Collection of Samples

2.2.1 A sample, consisting of ten sample units drawn at random from each lot, shall be taken.

2.2.2 Each sample unit shall consist of at least 100 ml.

2.2.3 Collect original unopened containers wherever possible.

2.2.4 Ship and store the sample units under refrigeration (<5°C) if more than 2 hr elapse between collection and analysis. Do not freeze the sample units.