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Food and Nutrition

HPB Methods for the Microbiological Analysis of Foods

Table of Contents - Volume 2 - The Compendium of Analytical Methods
Identification Number Title Date Issued
Disclaimer Disclaimer for the Compendium of Analytical Methods January 2005
MFHPB-01 Determination of Commercial Sterility and the Presence of Viable Microorganisms in Canned Foods March 2001
Supplement to the Method MFHPB-01 October 2001
MFHPB-02 Method for the Direct Microscopic Examination of Foods in Hermetically Sealed Containers October 2006
MFHPB-03 Determination of the pH of foods including foods in hermetically sealed containers February 2003
MFHPB-04 Method to Measure the Internal Pressure (Negative or Positive) of Foods in Hermetically Sealed Rigid Containers February 2003
MFHPB-05 Method for the Determination of Micro-leaks in Hermetically Sealed Metal and Glass Containers May 2003
MFHPB-06 Method for Examination and Evaluation of Hermetically Sealed Metal Cans and Glass Container May 2003
MFHPB-07 The isolation of Listeria monocytogenes and other Listeria spp. from foods and environmental samples using Palcam broth March 2012
MFHPB-16 Detection of Clostridium botulinum and its Toxins in Suspect Foods and Clinical Specimens March 2009
MFHPB-17 Enumeration of Coliforms in Foods by the Hydrophobic Grid-Membrane Filter (HGMF) Method March 2001
MFHPB-18 Determination of the Aerobic Colony Count in Foods October 2001
MFHPB-19 Enumeration of Coliforms, Faecal Coliforms and of E. coli in Foods April 2002
MFHPB-20 Methods for the Isolation and Identification of Salmonella from Foods and Environmental Samples March 2009
MFHPB-20A Amendment to MFHPB-20 for the analysis of seeds used to manufacture sprouts Archived March 2009
MFHPB-21

Enumeration of Staphylococcus aureus in Foods

September 2005

MFHPB-22 Enumeration of Yeasts and Molds in Foods January 2004
MFHPB-23 Enumeration of Clostridium perfringens in Foods November 2001
MFHPB-24 Detection of Salmonella Spp. In Foods by the Vidas SLM™ Method November 2001
MFHPB-25 Methods for the Evaluation of Canned Foods and Their Containers See MFHPB-01 to MFHPB-06
MFHPB-26 Enumeration of Escherichia coli in Foods by the Hydrophobic Grid-Membrane Filter Method (HGMF) Method March 2001
MFHPB-27 Enumeration of Escherichia coli in Foods by the Direct Plating (DP) Method September 1997
MFHPB-28 Determination of Staphylococcus aureus Thermostable Nuclease in Foods.
The figures for this method are available in hard-copy from the Editor
May 2001
MFHPB-29 Detection of Listeria spp. In Foods and Environmental Samples by the Vidas Listeria™ Method March 2012
MFHPB-30 Isolation of Listeria monocytogenes and other Listeria spp. from foods and environmental samples April 2011
MFHPB-31 Determination of Coliforms in Foods Using Violet Red Bile Agar March 2001
MFHPB-32 Enumeration of Yeast and Mold in Food Products and Food Ingredients Using 3M™ Petrifilm™ Yeast and Mold Count Plates March 2003
MFHPB-33 Enumeration of Total Aerobic Bacteria in Food Products and Food Ingredients Using 3M™ Petrifilm™ Aerobic Count Plates February 2001
MFHPB-34 Enumeration of E scherichia coli and Coliforms in Food Products and Food Ingredients Using 3M™ Petrifilm™ E. coli Plates September 2012
MFHPB-35 Enumeration of Coliforms in Food Products and Food Ingredients Using 3M™ Petrifilm™ Coliform Count Plates February 2001
MFHPB-36 No Method Yet  
Appendix A Definitions of Terms November 2006
Appendix B Collection and Transportation of Samples November 2006
Appendix C Calculation of the Most Probable Number of Growth Units for HGMF Methods March 1993
Appendix D Calculation of the Most Probable Number (MPN) March 1993
Appendix E Enzyme Treatment of Foods for Filtration September 1993
Appendix F Supplement to all the Methods in the Compendium:
the use of a Stomacher vs a blender
November 1999
Appendix G  Glossary of Media and Reagents  
Index and Introduction February 2006
Supplement to the Media Glossary February 2004
Section A January 2006
Section B January 2006
Section C January 2006
Section D January 2006
Section E January 2006
Section F January 2006
Section G January 2006
Section H January 2006
Section I January 2006
Sections J-K January 2006
Section L January 2006
Section M January 2006
Section N January 2006
Section O January 2006
Section P February 2006
Sections Q-R January 2006
Section S January 2008
Section T February 2006
Section U January 2006
Section V January 2006
Section W January 2006
Sections X-Y January 2006
Appendix H Preparation of Dilutions using Gravimetric Means December 2001
Appendix I Supplement to All Methods in the Compendium: General Microbiological Guidance

Supplement to Appendix I
January 2003

June 2005
Appendix J Supplement to All Methods That Detect Salmonella November 2003
Appendix K Summaries of Methods in this Compendium that Detect Specific Bacteria in this Compendium  
E. coli O157:H7 March 2012
Listeria spp. and L. monocytogenes March 2012
Staphylococcus aureus March 2012
Salmonella spp. March 2012
Appendix L Confirmation Steps for Methods for The Detection of Listeria spp. In Foods And Environmental Samples Archived July 2012

Evaluation Division
Bureau of Microbial Hazards
Food Directorate
Health Products and Food Branch
Health Canada
4th Floor N.W. SFBRC [2204A1]
Ottawa, Ontario K1A 0L2
Telephone: (613) 957-0349
Fax: (613) 952-6400
Micro_methods_committee@hc-sc.gc.ca