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Laboratory Procedures for the Microbiological Analysis of Foods

The Laboratory Procedures for the Microbiological Analysis of Foods are available upon request. Please select the appropriate link in the table below to send your request via email, or contact Health Canada's publication office to make your request.

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Table of Contents - Volume 3 - The Compendium of Analytical Methods
Identification Number Title Date Issued
Disclaimer Disclaimer for the Compendium of Analytical Methods January 2005
MFLP-02 Detection of Shiga toxins of Shiga toxin-producing Escherichia coli (STEC) by the Pro-Lab ProlisaTM STEC Enzyme Immunoassay Kit July 2016
MFLP-03

The Atlas® E. coli O157:H7 EG2 Detection Assay for detection of Escherichia coli O157:H7 in raw beef trim

June 2016
MFLP-05 Detection of Listeria spp. from environmental surfaces using the 3M™ Molecular Detection System Test Kit November 2013
MFLP-06 Detection of Salmonella spp. in Foods using the 3M™ Molecular Detection System Test Kit July 2013
MFLP-07 The detection of Listeria monocytogenes in foods and environmental surfaces using the Assurance GDS™ Listeria monocytogenes Tq Genetic Detection System October 2014
MFLP-08 The detection of Listeria species in foods and environmental surfaces using the Assurance GDS« Listeria spp. Tq Genetic Detection System November 2015
MFLP-09 Enumeration of Enterobacteriaceae species in Food and Environmental Samples Using 3M™ Petrifilm™ Enterobacteriaceae Count Plates June 2007
MFLP-10 Enumeration of Total Aerobic Bacteria in Food Products and Food Ingredients Using Compact Dry Aerobic Count Plates December 2007
MFLP-11 Enumeration of Listeria species in Environmental Samples Using 3M™ Petrifilm™ Environmental Listeria Plates February 2011
MFLP-12 Identification of Escherichia coli O157:H7 and Verotoxin-Producing Escherichia coli O157:NM using the ADIAFOOD Rapid Pathogen Detection System, a Real-Time PCR Technique March 2009
MFLP-13 The Genequence Listeria spp. Assay for the Detection of Listeria in Foods and Environmental Surfaces February 2011
MFLP-14 The Genequence Listeria monocytogenes Assay for the Detection of Listeria monocytogenes in a Variety of Foods October 2013
MFLP-15 The Detection of Listeria species from Environmental Surfaces Using the Dupont Qualicon BAX« System Method and Direct Plating February 2011
MFLP-16 Detection of Escherichia coli O157:H7 in foods - Assurance GDS® for E. coli O157:H7 Tq Gene Detection System December 2015
MFLP-17 Enumeration of Viable Aerobic Mesophilic Microorganisms in Food Products using the TEMPO« TVC February 2010
MFLP-18 The 48 h Dupont™ Lateral Flow System™ Method for the Detection of Salmonella species in Raw and Processed Meats Removed May 2015
MFLP-19 The Dupont™ Lateral Flow System™ Method for Detecting E. coli O157 in Raw Ground and Raw Boneless Beef Removed December 2013
Supplement to Method MFLP-19 Removed December 2013
MFLP-20 The GeneQuence® Salmonella Microwell Assay for the detection of Salmonella spp. March 2016
MFLP-21 Enumeration of Staphylococcus aureus in Foods and Environmental Samples Using 3MT PetrifilmT Staph Express Count (STX) Plates July 2004
Supplement to Method MFLP-21 Removed April 2013
MFLP-22 Characterization of Verotoxigenic Escherichia coli O157:H7 Colonies by Polymerase Chain Reaction (PCR) and Cloth-Based Hybridization Array System (CHAS) December 2013
MFLP-23 Specific Detection of Vibrio parahaemolyticus Strains using a Multiplex Polymerase Chain Reaction (PCR) Based on the R72H Taxonomic Marker and the Hemolysin Genes TDH and TRH March 2006
Figure
MFLP-24 Identification of Escherichia coli O157 by the Warnex Real Time Polymerase Chain Reaction System Removed April 2013
MFLP-25 Isolation and Identification of Shigella spp. from Foods March 2006
MFLP-26 Detection of Shigella spp. in Foods by the Polymerase Chain Reaction (PCR) February 2006
MFLP-27 The Dupont Qualicon Bax« System Method for the Detection of Enterobacter sakazakii in Selected Foods September 2003
MFLP-28 The Qualicon Bax« System Method for the Detection of Listeria monocytogenes in a Variety of Food November 2011
MFLP-29 The DuPont BAX® System Method for the detection of Salmonella in foods and environmental surface samples March 2016
MFLP-30 Detection of Escherichia coli O157:H7 in Select Foods using the BAX« System E. coli O157:H7 MP November 2012
MFLP-31 Identification of Listeria monocytogenes using the ADIAFOOD Rapid Pathogen Detection System, a Real-Time PCR Technique October 2011
MFLP-32 Detection of Salmonella spp. using the ADIAFOOD Rapid Pathogen Detection System, A Real-Time PCR Technique March 2016
MFLP-33 Detection of Listeria monocytogenes in Foods by the VIDAS LMO 2™ Method August 2014
MFLP-34 Detection of Listeria spp. in foods and environmental surface samples by the VIP Gold method September 2015
MFLP-35 Detection of Salmonella by the TECRA Salmonella Visual Immunoassay (VIA) Method Removed November 2014
MFLP-36 Detection of Salmonella in Foods and Environmental Surface Samples - Assurance GDS® for Salmonella Tq Genetic Detection System March 2016
MFLP-37 Part 1: Detection of Halophilic Vibrio species in Seafood

Part 2: Detection of Vibrio cholerae
October 2006
MFLP-38 Detection of Salmonella spp. from all foods and selected environmental surfaces using iQ-Check™ Salmonella Real-Time PCR Test Kit August 2012
MFLP-39 Detection of Listeria spp. from Environmental Surfaces and Heat Processed Ready to Eat Meat and Poultry Using iQ-Check™ Listeria spp. Real-Time PCR Test Kit May 2013
MFLP-40 Detection of Salmonella in Food Products by the VIDAS« Easy Salmonella (SLM) Method November 2011
MFLP-41 Environmental Sampling for the Detection of Microorganisms July 2010
MFLP-42 Isolation and Enumeration of the Bacillus cereus Group in Foods May 2011
MFLP-43 Determination of Enterobacteriaceae September 1997
MFLP-44 Determination of Aerobic and Anaerobic Sporeformers March 2012
MFLP-45 Determination of Sodium Chloride (Salt) in Foods and Other Samples by Quantab™ Chloride Titrators November 2007
MFLP-46 Isolation of thermophilic Campylobacter from Food December 2014
MFLP-47 Determination of Enterotoxin Production by Staphylococcus aureus Isolated from Food (microslide gel double diffusion assay) Removed April 2012
MFLP-48 Isolation of Yersinia enterocolitica from Foods and Environmental Samples May 2012
MFLP-49 Detection of Salmonella spp. in food products and environmental surfaces by the VIDAS® UP Salmonella (SPT) method August 2014
MFLP-50 Detection of Clostridium botulinum in Honey and Syrups January 2012
MFLP-51 Detection of Listeria species in Stainless steel Surfaces by the ADIAFOOD Rapid Pathogen Detection System July 2012
MFLP-52 Isolation and identification of priority verotoxigenic Escherichia coli (VTEC) in foods November 2014
MFLP-53 Identification of Listeria monocytogenes colonies by polymerase chain reaction (PCR) and cloth-based hybridization array system (CHAS) August 2015
MFLP-54 Detection of Salmonella spp. in Food and Agricultural Products by the Gene-Trak« DNA Hybridization Method Removed April 2012

MFLP-55

Enumeration of Faecal Coliforms in Foods by the Hydrophobic Grid-Membrane Filter (HGMF) Method March 2015
MFLP-56 Determination of Aerobic Colony Count in Foods and Environmental Samples by the Hydrophobic Grid-Membrane Filter (HGMF) Method January 2003
MFLP-57 Enumeration of Yeast and Mold in Foods Using the Iso-Grid Membrane Filtration System November 2014
MFLP-58 Enumeration of Aeromonas hydrophila in Foods by Hydrophobic Grid-Membrane Filter or Direct Plating December 2014
MFLP-58B Enumeration of Aeromonas hydrophila in Ice and Water by the Hydrophobic Grid-Membrane Filter (HGMF) Technique October 2012
MFLP-59 Detection of Listeria spp. in food products and environmental surface samples with VIDAS« UP Listeria (LPT) June 2016
MFLP-60 Screening Bottled Water (Water in Sealed Containers) for the Presence of Indicator and Pathogenic Bacteria Removed April 2012
MFLP-61 Enumeration of Pseudomonas aeruginosa in Foods and Food Ingredients by the Hydrophobic Grid-Membrane Filter (HGMF) Method May 2015
MFLP-61B Enumeration of Pseudomonas aeruginosa in prepackaged ice and water in sealed containers by the Hydrophobic Grid-Membrane Filter (HGMF) Technique November 1999
MFLP-62 Determination of Verotoxin genes in Verotoxigenic Escherichia coli isolates November 2015
MFLP-63 Measuring Water Activity (aw) Using the Novasina aw Center January 2006
MFLP-64 Analysis of Sprouts for Coliforms, Escherichia coli, and Klebsiella pneumoniae. Removed April 2012
MFLP-65 Detection of Staphylococcal Enterotoxins in Food Products Using the VIDAS« Staph Enterotoxin II (SET2), an ELFA (Enzyme Linked Fluorescent Assay) Technique April 2008
MFLP-66 Determination of Water Activity Using the Decagon Aqualab August 2014
MFLP-67 Determination of Enterotoxin Production by Staphylococcus aureus in Foods and Broth Media [The Reversed Passive Latex Agglutination (RPLA) Method] Removed July 2012
MFLP-68 Detection of Enterotoxins Produced by Staphylococcus aureus in Foods Enzyme-linked Immunosorbent Assay (ELISA) using the Tecra Kit April 1998
MFLP-69 Detection of Enterotoxins Produced by Staphylococcus aureus (Ridascreen SET A, B, C, D, E Enzyme Immunoassay) Removed April 2014
MFLP-70 Detection of Salmonella in Foods by the Modified 1-2 Test Removed November 2012
MFLP-71 Detection of Listeria spp. in Foods and Environmental Samples using the Oxoid Listeria Rapid Test December 2013
MFLP-72 The Isolation and Identification of Vibrio cholerae O1 and non-O1 from Foods Removed April 2012
MFLP-73 The Isolation and Enumeration of Vibrio vulnificus from Fish and Seafoods Removed April 2012
MFLP-74 Enumeration of Listeria monocytogenes in foods February 2011
MFLP-75 Procedure for the Isolation of Salmonella species by the Modified Semi-solid Rappaport Vassiliadis (MSRV) Method March 2016
MFLP-76 The DuPont Qualicon BAX« System real-time method for the detection of E. coli O157:H7 in raw beef trim and raw ground beef November 2014
MFLP-77 Detection of Listeria monocytogenes and other Listeria spp. in Food Products and Environmental Samples by the VIDAS« Listeria species Xpress (LSX) Method October 2012
MFLP-78 Identification of Listeria monocytogenes from foods and environmental samples by the Polymerase Chain Reaction (PCR) February 2011
MFLP-79 Identification Of Staphylococcus aureus by Accuprobe™(Accuprobe Rapid DNA Probe Test) Removed April 2012
MFLP-80 Isolation of E. coli O157:H7 or NM in Foods See MFHPB-10
MFLP-81 Detection of Enterohemorrhagic E. coli (EHEC) in Food Products and Food Ingredients by the Assurance EHEC Enzyme Immunoassay (EIA) Method Removed July 2013
MFLP-82 Detection of E. coli O157 by the Merck Singlepath« E. coli O157 Kit July 2014
MFLP-83 Detection of Verotoxins VT1 and VT2 from Escherichia coli O157:H7/NM by the Merck Duopath« Verotoxin Kit January 2015
MFLP-84 Method for the ImmunoMagnetic Separation of Salmonella species by DynabeadsTM anti-Salmonella March 2016
MFLP-85 Enumeration of Coliforms in Foods and Environmental Samples Using 3M ™ Petrifilm™ High Sensitivity Coliform Count (HSCC) Plates March 2003
MFLP-86 Identification of vt1 and vt2 genes from Verotoxigenic Escherichia coli by Polymerase Chain Reaction November 2014
MFLP-87 Detection of Enterohemorrhagic Escherichia coli O157 (EHEC) in Food Products and Food Ingredients by the VIP® Gold for EHEC Method September 2016
MFLP-88

Identification of Listeria monocytogenes by AccuProbe™ (AccuProbe Rapid DNA Probe Test)

July 2011
MFLP-89 Procedure for the Detection of Verocytotoxin-Producing Escherichia coli in Food Samples Removed April 2012
MFLP-90 Identification of E. coli O157 by DynaBeads™ anti-E. coli O157 See MFHPB-10
MFLP-91 Detection of E. coli O157 by the TECRA™ E. coli O157 Visual Immunoassay™ (VIA) Method and TECRA E.coli O157 Immunocapture Removed May 2014
MFLP-92 Detection of Escherichia coli O157 in Foods by Polymyxin-based Enzyme-linked Immunosorbent Assay Removed April 2013
MFLP-93 Identification of Escherichia coli Verotoxins by the Meridian Premier EHEC Kit™ Removed August 2013
MFLP-94 The Eight Hour Reveal Method for Detecting Escherichia coli O157:H7 In Raw Beef and Environmental Samples. Removed January 2014
MFLP-95 The 20 Hour Reveal Method for Detecting Escherichia coli O157:H7 from Foods and Environmental Samples. Removed January 2014
MFLP-96 The Reveal Test Kit for Detecting Salmonella Removed December 2012
MFLP-97 The Alert Test Kit for Detecting Salmonella Removed December 2013
MFLP-98 Detection of E. coli O157:H7 in Food Products by the VIDAS« UP E. coli O157 (including H7) Method July 2015
MFLP-99 Detection of Listeria spp. in environmental samples using FoodChek™ Listeria spp. method January 2015

Bureau of Microbial Hazards, Evaluation Division, Food Directorate