Laboratory Procedures for the Microbiological Analysis of Foods

• Volume 1
• Volume 2
• Volume 3
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• Volume 5

Table of Contents - Volume 3 - The Compendium of Analytical Methods

Identification Number	Title	Date Issued
Disclaimer	Disclaimer for the Compendium of Analytical Methods	January 2005
MFLP-07	The detection of Listeria monocytogenes in foods and environmental surfaces using the Assurance GDS™ Listeria monocytogenes Genetic Detection System	June 2012
MFLP-08	The detection of Listeria species in foods and environmental surfaces using the Assurance GDS™ Listeria spp. Genetic Detection System	June 2012
MFLP-09	Enumeration of Enterobacteriaceae Species in Food and Environmental Samples Using 3M™ Petrifilm™ Enterobacteriaceae Count Plates	June 2007
MFLP-10	Enumeration of Total Aerobic Bacteria in Food Products and Food Ingredients Using Compact Dry Aerobic Count Plates	December 2006
MFLP-11	Enumeration of Listeria Species in Environmental Samples Using 3M™ Petrifilm™ Environmental Listeria Plates	April 2011
MFLP-12	Identification of Escherichia coli O157:H7 and Verotoxin-producing Escherichia coli O157:NM by the ADIAFOOD Rapid Pathogen Detection System, a Real-time Polymerase Chain Reaction System	March 2009
MFLP-13	The Genequence Listeria spp. Assay for the Detection of Listeria in a Variety of Foods and Environmental Surfaces	April 2011
MFLP-14	The Genequence Listeria Monocytogenes Assay for the Detection of Listeria monocytogenes in a Variety of Foods	April 2011
MFLP-15	The Detection of Listeria Species from Environmental Surfaces Using the Dupont Qualicon BAX® System Method and Direct Plating	April 2011
MFLP-16	Detection of Escherichia coli O157:H7 in Foods - Assurance GDS™ for E. coli O157:H7 Gene Detection System	August 2005
MFLP-17	Enumeration of viable aerobic mesophilic microorganisms in food products using the TEMPO® TVC	February 2010
MFLP-18	The 48 h Dupont™ Lateral Flow System™ Method for the Detection of Salmonella species in Raw and Processed Meats	November 2007
MFLP-19	The Dupont™ Lateral Flow System™ Method for Detecting E. coli O157 in Raw Ground and Raw Boneless Beef	March 2006
	Supplement to Method MFLP-19	August 2007
MFLP-20	The Genequence® Salmonella Microwell Assay for the Detection of Salmonella in a Variety of Foods	October 2004
MFLP-21	Enumeration of Staphylococcus Aureus in Foods and Environmental Samples Using 3MT PetrifilmT Staph Express Count (STX) Plates	July 2004
	Supplement to Method MFLP-21	Archived April 2013
MFLP-23	Specific Detection of Vibrio Parahaemolyticus Strains Using a Multiplex Polymerase Chain Reaction (PCR) Based on the R72H Taxonomic Marker and the Hemolysin Genes TDH and TRH	March 2006
	Figure	March 2006
MFLP-24	Identification of Escherichia coli O157 by the Warnex Real Time Polymerase Chain Reaction System	Archived April 2013
MFLP-25	Isolation and Identification of Shigella Spp. From Foods	March 2006
MFLP-26	Detection of Shigella Spp. In Foods by the Polymerase Chain Reaction (PCR)	February 2006
MFLP-27	The Dupont Qualicon Bax® System Method for the Detection of Enterobacter Sakazakii in Selected Foods	September 2003
MFLP-28	The Qualicon Bax® System Method for the Detection of Listeria Monocytogenes in a Variety of Food	November 2011
MFLP-29	The Qualicon Bax® System Method for the Detection of Salmonella in a Variety of Food and Environmental Samples	June 2012
MFLP-30	The Dupont Qualicon Bax® System Method for the Detection of E. Coli O157:H7 in Raw Beef and Fruit Juice	May 2003
	Supplement to MFLP-30	May 2005
	Supplement 2 to MFLP-30 The Use of the Bax E. coli O157:H7 MP Assay	November 2006
MFLP-31	Identification of Listeria monocytogenes using the Adiafood Rapid Pathogen Detection System, a Real-Time PCR Technique	October 2011
MFLP-32	Identification of Salmonella species using the Adiafood rapid pathogen detection system, a real-time pcr technique	January 2010
MFLP-33	Detection of Listeria monocytogenes in foods by the VIDAS LMO 2™ method	March 2012
MFLP-34	Detection of Listeria spp. in Foods and Environmental Samples by the VIP Method	Under Review
MFLP-35	Detection of Salmonella by the Tecra Salmonella Visual Immunoassay (Via) Method	June 2002
MFLP-36	Detection of Salmonella in Foods and Environmental Surfaces - Assurance GDS for Salmonella Gene Detection System	June 2006
MFLP-37	Part 1: Detection of Halophilic Vibrio Species in Seafood Part 2: Detection of Vibrio Cholerae	October 2006
MFLP-38	Detection of Salmonella spp. from all foods and selected environmental surfaces using iQ-Check™ Salmonella Real-Time PCR Test Kit	August 2012
MFLP-39	Detection of Listeria spp. From Environmental Surfaces Using iQ-CheckTM Listeria spp. Real-Time PCR Test Kit	April 2011
MFLP-40	Detection of Salmonella in food products by the VIDAS® Easy Salmonella (SLM) method	November 2011
MFLP-41	Environmental Sampling for the Detection of Microorganisms	July 2010
MFLP-42	Isolation and Enumeration of the Bacillus Cereus Group in Foods	May 2011
MFLP-43	Determination of Enterobacteriaceae	September 1997
MFLP-44	Determination of Aerobic and Anaerobic Sporeformers	April 1998
	Supplement to Method MFLP-44	September 1999
MFLP-45	Determination of Sodium Chloride (Salt) in Foods and Other Samples by Quantab™ Chloride Titrators	November 2007
MFLP-46	Isolation of Thermophilic Campylobacter from Foods	March 2002
MFLP-47	Determination of Enterotoxin Production by Staphylococcus aureus Isolated from Food (microslide gel double diffusion assay)	Archived April 2012
MFLP-48	Isolation of Yersinia enterocolitica and from Foods and Environmental Samples	May 2012
MFLP-49	Detection of Salmonella spp. In Food and Agricultural Products by the Vidas SLM Method.	( See mfhpb-24)
MFLP-50	Detection of Clostridium botulinum in Honey and Syrups	January 2012
MFLP-51	Detection of Listeria species in Stainless steel Surfaces by the ADIAFOOD Rapid Pathogen Detection System	July 2012
MFLP-52	Identification of Staphylococcus aureus in Foods by the Warnex Semi-quantitative Real-time Polymerase Chain Reaction System	Archived March 2009
MFLP-53	Identification of Escherichia Coli in Foods by the Warnex™ Semi-quantitative Real-time Polymerase Chain Reaction System	Archived March 2009
MFLP-54	Detection of Salmonella spp. In Food and Agricultural Products by the Gene-Trak® DNA Hybridization Method	Archived April 2012
MFLP-55	Enumeration of Faecal Coliforms in Foods by the Hydrophobic Grid-Membrane Filter (HGMF) Method	September 1998
MFLP-56	Determination of Aerobic Colony Count in Foods and Environmental Samples by the Hydrophobic Grid-Membrane Filter (HGMF) Method	January 2003
MFLP-58	Enumeration of Aeromonas hydrophila by the Hydrophobic Grid-Membrane Filter (HGMF) Technique	Under review
MFLP-58B	Enumeration of Aeromonas hydrophila in prepackaged ice and water in sealed containers by the Hydrophobic Grid-Membrane Filter (HGMF) Technique	November 1999
MFLP-60	Screening Bottled Water (Water in Sealed Containers) for the Presence of Indicator and Pathogenic Bacteria.	Archived April 2012
MFLP-61	Enumeration of Pseudomonas aeruginosa in Foods and Food Ingredients by the Hydrophobic Grid-Membrane Filter (HGMF) Method	May 2001
MFLP-61B	Enumeration of Pseudomonas aeruginosa in prepackaged ice and water in sealed containers by the Hydrophobic Grid-Membrane Filter (HGMF) Technique	November 1999
MFLP-62	The Qualicon Bax® Method for the Detection of Salmonella In Foods and Environmental Samples	Archived: December 2003 See MFLP-29
MFLP-63	Measuring Water activity (aW) Using the Novasina aW Center	January 2006
MFLP-64	Analysis of sprouts for coliforms, Escherichia coli, and Klebsiella pneumoniae.	Archived April 2012
MFLP-65	Detection of Staphylococcal Enterotoxins in Food Products Using the VIDAS® Staph Enterotoxin II (SET2), an ELFA (Enzyme Linked Fluorescent Assay) Technique	April 2008
MFLP-66	Determination of Water Activity Using the Decagon Aqualab CX-2 and Series 3	June 2006
MFLP-67	Determination of Enterotoxin Production by Staphylococcus Aureus in Foods and Broth Media [The Reversed Passive Latex Agglutination (RPLA) Method]	Archived July 2012
MFLP-68	Detection of Enterotoxins Produced by Staphylococcus aureus in Foods (The Enzyme-linked Immunosorbent Assay (ELISA) using the Tecra Kit)	April 1998
MFLP-69	Detection of Enterotoxins Produced by Staphylococcus aureus in Foods (Ridascreen SET A, B, C, D, E Enzyme Immunoassay)	April 1998
MFLP-70	Detection of Salmonella in Foods by the Modified 1-2 Test	Archived November 2012
MFLP-71	Detection of Listeria spp. in Foods Using the Oxoid Listeria Rapid Test (The Clearview Kit)	April 2011
MFLP-72	The Isolation and Identification of Vibrio cholerae 01 and non-01 from Foods	Archived April 2012
MFLP-73	The Isolation and Enumeration of Vibrio vulnificus from Fish and Seafoods	Archived April 2012
MFLP-74	Enumeration of Listeria monocytogenes in Food	April 2011
MFLP-75	Procedure for the Isolation of Salmonella species by the Modified Semi-solid Rappaport Vassiliadis (MSRV) Method	June 2004
	Supplement to Method MFLP-75	February 2006
MFLP-76	The DuPont Qualicon BAX® System real-time method for the detection of E. coli O157:H7 in raw beef trim and raw ground beef	April 2011
MFLP-77	Detection of Listeria monocytogenes and other Listeria spp. in food products and environmental samples by the VIDAS® Listeria species Xpress (LSX) method	October 2012
MFLP-78	Identification of Presumptive Positive Listeria monocytogenes From Foods and environmental Samples by the Polymerase Chain Reaction (PCR)	April 2011
MFLP-79	Identification Of Staphylococcus aureus by Accuprobe™(Accuprobe Rapid DNA Probe Test)	Archived April 2012
MFLP-80	Isolation of E. coli O157:H7 or NM in Foods	March 2008
MFLP-81	Detection of Enterohemorrhagic E. coli (EHEC) in Food Products and Food Ingredients by the Assurance EHEC Enzyme Immunoassay (EIA) Method	June 2006
MFLP-82	Detection of E. coli O157 by the Merck Singlepath® E. coli O157 Kit	July 2007
MFLP-83	Detection of Verotoxins VT 1 and VT 2 by the Merck Duopath® Verotoxin Kit	December 2006
MFLP-84	Identification of Salmonella spp. by Dynabeads anti-Salmonella	December 2006
MFLP-85	Enumeration of Coliforms in Foods and Environmental Samples Using 3M ™ Petrifilm™ High Sensitivity Coliform Count (HSCC) Plates	March 2003
MFLP-86	Identification of Presumptive Positive Verocytoxigenic Escherichia coli by the Polymerase Chain Reaction	January 2003
MFLP-87	Detection of Enterohemorrhagic E. coli (EHEC) in Food Products and Food Ingredients by the VIP for EHEC Method	Under Review
MFLP-88	Identification of Listeria monocytogenes by Accuprobe™ (Accuprobe Rapid DNA Probe Test)	July 2011
MFLP-89	Procedure for the Detection of Verocytotoxin-Producing Escherichia coli in Food Samples	Archived April 2012
MFLP-90	Identification of E. coli O157 by DynaBeads™ anti-E. coli O157	July 2006
MFLP-91	Detection of E. coli O157 by the Tecra™ E. coli O157 Visual ImmunoassayTM (VIA) Method and Tecra E.coli O157 Immunocapture	February 2006
MFLP-92	Detection of Escherichia coli O157 in Foods by Polymyxin-based Enzyme-linked Immunosorbent Assay	Archived April 2013
MFLP-93	Identification of Escherichia coli Verotoxins by the Meridian Premier EHEC Kit™	November 2005
MFLP-94	The Eight Hour Reveal Method for Detecting Escherichia coli O157:H7 In Raw Beef and Environmental Samples.	September 2000
MFLP-95	The 20 Hour Reveal Method for Detecting Escherichia coli O157:H7 from Foods and Environmental Samples.	September 2000
MFLP-96	The Reveal Test Kit for Detecting Salmonella	Archived December 2012
MFLP-97	The Alert Test Kit for Detecting Salmonella	February 2001
MFLP-98	Detection of E. coli O157:H7 in food products by the VIDAS® UP E. coli O157 (including H7) method	December 2011
Appendix A	Definition of Terms - Applicable to all Methods in the Compendium of Analytical Methods	November 2006
Appendix B	Collection and Transportation of Samples - Applicable to all Methods in the Compendium of Analytical Methods	November 2006
Appendix C	Calculation of the Most Probable Number of Growth Units for HGMF Methods	March 1993
Appendix D	Calculation of the Most Probable Number (MPN)	March 1993
Appendix E	Enzyme Treatment of Foods for Filtration	September 1993
Appendix F	Supplement to all the Methods in the Compendium: the use of a Stomacher vs a blender	November 1999
Appendix G	Glossary of Agars, Broths and Other Reagents
	Index and Introduction	February 2006
	Section A	January 2006
	Section B	January 2006
	Section C	January 2006
	Section D	January 2006
	Section E	January 2006
	Section F	January 2006
	Section G	January 2006
	Section H	January 2006
	Section I	January 2006
	Sections J-K	January 2006
	Section L	January 2006
	Section M	January 2006
	Section N	January 2006
	Section O	January 2006
	Section P	February 2006
	Sections Q-R	January 2006
	Section S	January 2008
	Section T	February 2006
	Section U	January 2006
	Section V	January 2006
	Section W	January 2006
	Sections X-Y	January 2006
Appendix H	Preparation of Dilutions using Gravimetric Means	December 2001
Appendix I	Supplement to All Methods in the Compendium: General Microbiological Guidance
	Supplement to Appendix I	June 2005
Appendix J	Supplement to All Methods That Detect Salmonella	November 2003
Appendix K	Summaries of Methods that Detect Specific Bacteria in this Compendium
	E. coli O157:H7	March 2012
	Listeria spp. and L. monocytogenes	March 2012
	Staphylococcus aureus	March 2012
	Salmonella spp.	March 2012
Appendix L	Confirmation Steps for Methods for The Detection of Listeria spp. In Foods And Environmental Samples	Archived July 2012

Evaluation Division
Bureau of Microbial Hazards
Food Directorate
Health Products and Food Branch
Health Canada
4th Floor N.W. SFBC [2204E]
Ottawa, Ontario K1A 0K9
Telephone: 613-957-0349
Facsimile: 613-952-6400
Micro_methods_committee@hc-sc.gc.ca