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Detection of Salmonella in Foods by the Modified 1-2 Test
Laboratory Procedure MFLP-70
April 1995
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(PDF Version - 67 K)Health Protection Branch
Ottawa
Don Warburton1 and John Oggel2
1Bureau of Microbial Hazards, Food Directorate, Ottawa, Ontario, K1A 0L2
2Laboratory Services, Agri-Food and Agriculture Canada, Ottawa, Ontario, K1A 0C6
1. Application
The method is applicable to the detection of viable motile Salmonella from feeds, raw foods and other samples. It is a suitable screening test, especially where follow up product action is not anticipated. When product-based compliance action is anticipated, and where stipulated, the Official Methods and HPB Method should be used. This revised method replaces MFLP-70, dated April 1994.
2. Description
The method has been shown to produce satisfactory results with naturally-contaminated animal feed, chicken, pork, seafood and artificially-contaminated foods in AOAC and HPB studies (8.3-8.6). This method can be used successfully for the detection of motile Salmonella in other foods, food ingredients, and environmental samples.
3. Principle
Following pre-enrichment and selective enrichment in tetrathionate brilliant green broth, the sidearm of the 1-2 Test is inoculated. Motile Salmonella grow through semi-solid medium and react with polyvalent H (flagellar) antibodies to form an immunoband. This presumptive identification requires 48 h to complete. It is not a confirmatory test because the polyvalent H antibodies may cross-react with a small percentage of non-Salmonella. NB: This method does not detect non-motile salmonellae. Pathogenic, non-motile strains have not been implicated in foodborne disease(s) since 1964 (8.5; E.C.D. Todd; pers. com.). Nonmotile strains represent <1% of isolates from clinical samples and animal feeds (J. Oggel, AAFC and H. Lior, HPB; pers. com.). Confirmatory biochemical and serological tests are performed on isolated colonies.
4. Definition of Terms
See Appendix A of Volume 3.
5. Collection of Samples
See Appendix B of Volume 3.
6. Materials and Special Equipment
1) 1-2 Test (BioControl Systems Inc., phone: (206) 487-2055, FAX: (206) 487-1476)
2) Buffered Peptone Water (commercially available; Unipath, Ottawa)
3) Nutrient broth (commercially available)
