Supplement to the Method MFLP-75

Supplement to MFLP-75
February 2006

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Microbiological Methods Committee
Microbiology Evaluation Division
Bureau of Microbial Hazards, Food Directorate,
Health Products and Food Branch, Health Canada
Postal Locator: 2204A1
Ottawa, Ontario K1A 0L2

E-mail: Don_Warburton@hc-sc.gc.ca

1. Application

The following information is offered as a supplement to Method MFLP-75, dated June 2004, and must be used with this method. The purpose of this supplement is to provide additional information (such as source(s) of materials, critical steps, confirmation steps, or helpful hints etc.).

7.3 Sample Analysis

7.3.5 Animal feeds or feed ingredients

Equilibrate the 25 g, 100 g (or sample weight) to maintain a 1:10 final dilution, in 2 volumes of NB or BPW for 1 h at 35°C. Add another 7 volumes of pre-enrichment broth and stomach.

7.3.6 Environmental samples (dry samples, dust, etc. or swatches)

Follow MFLP-41B (Table 2): add NB or BPW at 100 mL/unit (sponge) or 10 mL/unit (swabs). Incubate at 35°C for 18-24 h.

7.3.7 For other samples, see MFHPB-20, Table 3.