Supplement to Method MFLP-80

Supplement to MFLP-80
September 2006

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Health Products and Food Branch
Ottawa

Microbiological Methods Committee
Microbiology Evaluation Division
Bureau of Microbial Hazards, Food Directorate,
Health Products and Food Branch, Health Canada
Postal Locator: 2204A1
Ottawa, Ontario K1A 0L2

E-mail: Don_Warburton@hc-sc.gc.ca

1. APPLICATION

The following information is offered as a supplement to Method MFLP-80, dated February 2006, and must be used with this method. The purpose of this supplement is to provide additional information (such as source(s) of materials, critical steps, confirmation steps, or helpful hints, etc.).

6. MATERIALS AND SPECIAL EQUIPMENT

25) Rapid detection kits such as VIP (MFLP- 87), Singlepath E. coli (MFLP-82, publication pending), Tecra VIA (MFLP-91), Polymixin EIA (MFLP-92) or equivalent (see Appendix K of the Compendium).

26) Toxin detection kits such as Meridian Premier EHEC kit (MFLP-93 ), Duopath VT kit (MFLP-83 ) or equivalent.

7. PROCEDURE

7.6 Confirmation Steps

7.6.5 Refer to Table 1 for the additional required confirmation steps.

Do either: A) VT Toxin Test (When toxin testing is required, it is recommended that a Compendium method be used such as MFLP-93 or MFLP-83 (or equivalent) that confirms the presence of toxin or toxin genes.);

OR B) Bax E. coli O157:H7 MP (or equivalent);

OR C) H7 Agglutination (complete serological testing using H7 antisera following manufacturer's instructions).

If positive, proceed as a confirmed E. coli O157:H7 or NM.

Continue with the method MFLP-80.