The Reveal Test Kit for Detecting Salmonella

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Laboratory Procedure MFLP-96
February 2001

1. Application

The method is applicable to the detection of Salmonella from feeds, raw foods and other samples to determine compliance with Sections 4 and 7 of the Food and Drugs Act. Where stipulated, the Official Methods and HPB Method should be used.

2. Description

The method has been shown to produce satisfactory results with artificially contaminated and naturally contaminated foods in AFNOR studies (8.1).

3. Principle

Following selective enrichment in Rappaport-Vasiliadis broth, a portion of the sample enrichment is placed into the sample port of the Reveal Device initiating flow. The Reveal Device contains antibodies with high specificity to Salmonella antigens. These antibodies are bound to colloidal gold and, separately, to a solid support matrix. Any Salmonella antigen present will bind to the gold conjugated antibodies forming an antigen - antibody - chromogen complex. This complex flows across a lateral flow membrane and is subsequently bound by antibody immobilized on the membrane. This causes the gold conjugate to precipitate, forming a visible line and indicating a positive reaction. Proper test completion and flow is indicated by a control line which forms further up in the test window and verifies a valid test run. Absence of a control line invalidates the test. Record results at 10 minutes incubation time.

4. Definition of terms

See Appendix A of Volume 3.

5. Collection of samples

See Appendix B of Volume 3.

6. Materials and special equipment

The media listed under No. 2 to 7 are commercially available and are to be prepared and sterilized according to the manufacturer's instructions.

1) Reveal Device (Neogen Corporation, phone: (517) 372-9200, Fax: (517) 372-0108)

2) REVIVE broth (Neogen Corp., Lansing, MI)

3) Rappaport-Vasiliadis broth at 2X concentration (Neogen Corp., Lansing, MI)