7) Wash bottles

8) Strong magnet

9) Incubator (50EC)

10) Suction filtration apparatus with Buchner or Hirsch funnel

11) Ruled filter paper. Filter paper should be larger than funnel plate.

12) Ashless filter paper. Filter paper should be larger than funnel plate.

13) Emulsifier (Igepal CO 730; GAF Corp.)

14) 1 N HCl

15) Concentrated HCl

16) Concentrated NH₄OH

17) Pepsin (1:10,000 - Difco Laboratories or equivalent)

18) Triton X-114 (Sigma Chemicals)

19) 1% sodium lauryl sulfate solution

20) Alcohol, 95%

21) Petri dishes to suit size of filter paper used

22) Fine, non-magnetic probe (platinum or glass recommended)

23) Glycerol-95% ethanol mixture (1:1)

24) Stereoscopic microscope (10-30x)

25) US standard No. 230 sieve

26) Platinum crucible

27) Muffle oven

28) Desiccator

5.1.1 In a random fashion from a sample unit weigh 225 g. This 225 g constitutes an analytical unit.

5.1.2 Repeat step 5.1.1 for remaining two sample units.