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Summary of Methods that Detect E. Coli O157:H7 in this Compendium

Health Products and Food Branch - Ottawa
Method Summary

October 2006

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NOTE: The following is a summary of methods that detect E. coli O157:H7 in the Compendium of Analytical Methods to aid the Laboratory Supervisor and Analyst to choosing the appropriate methods for the analysis of their sample(s). It is NOT an indication that each method must be used for each sample.

It is the responsibility of the Lab Supervisor to ensure that the latest version of each method and Supplements (as appropriate) are being used.
Method Type Identification Number of Method and Supplements Title
Screening (confirmation required with MFLP-80) MFLP-12 Identification of Escherichia coli O157:H7 and Verotoxin-producing Escherichia Coli 0157:NM by the WarnexTM Real-time Polymerase Chain Reaction System
MFLP-16 Detection of Escherichia coli O157:H7 in Foods - Assurance GDSTM for E. coli O157:H7 Gene Detection System
MFLP-19 The DupontTM Lateral Flow SystemTM Method for Detecting E. coli O157 in Raw Ground and Raw Boneless Beef
MFLP-24 Identification of Escherichia coli O157 by the Genevision Real Time Polymerase Chain Reaction System
MFLP-30 The Dupont Qualicon Bax® System Method for the Detection of E. coli O157:H7 in Raw Beef and Fruit Juice
MFLP-81 Detection of Enterohemorrhagic E. coli (EHEC) in Food Products and Food Ingredients by the Assurance EHEC Enzyme Immunoassay (EIA) Method
MFLP-82 Detection of E. coli O157 by the Merck Singlepath® E. coli O157 Kit (publication pending)
MFLP-86 Identification of Presumptive Positive Verocytoxigenic Escherichia coli by the Polymerase Chain Reaction
MFLP-87 Detection of Enterohemorrhagic E. coli (EHEC) in Food Products and Food Ingredients by the VIP for EHEC Method
MFLP-91 Detection of E. coli O157 by the TecraTM E. coli O157 Visual Immunoassay (VIA) Method
MFLP-92 Detection of Escherichia Coli O157 in Foods by Polymyxin-based Enzyme-linked Immunosorbent Assay
Screening (confirmation included) MFLP-94 The Eight Hour Reveal Method for Detecting Escherichia coli O157:H7 In Raw Beef and Environmental Samples.
MFLP-95 The 20 Hour Reveal Method for Detecting Escherichia coli O157:H7 from Foods and Environmental Samples.
Isolation and Identification MFLP- 80 Isolation of E. coli O157 in Foods
MFLP- 90 Identification of E. coli O157 by DynaBeadsTM anti-E. coli O157
Serology MFLP- 80 Isolation of E. coli O157 in Foods
Verotoxin Testing MFLP-83 Detection of Verotoxins VT 1 and VT 2 by the Merck Duopath® Verotoxin Kit (publication pending)
MFLP- 89 Procedure for the Detection of Verocytotoxin-Producing Escherichia coli in Food Samples
MFLP- 93 Identification of Escherichia coli Verotoxins by the Meridian Premier EHEC KitTM
Date Modified: 2006-10-03

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